Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, 608 002, Tamilnadu, India.
Mol Cell Biochem. 2013 Dec;384(1-2):113-20. doi: 10.1007/s11010-013-1788-2. Epub 2013 Aug 23.
In recent years, the role of endothelial dysfunction (ED) and excessive oxidative stress in the development of cardiovascular diseases has been highlighted. The aim of the present study is to evaluate the effect of diosgenin, an antioxidant on chronic renal failure (CRF) induced vascular dysfunction. CRF was induced by feeding the rats with a diet containing 0.75 % adenine and diosgenin was given orally (everyday at the dose of 40 mg/kg). Isometric force measurement was performed on isolated aortic rings in organ baths. Levels of reduced glutathione (GSH), nitric oxide metabolites, and endothelial nitric oxide synthase mRNA in rat aorta were examined. Further, plasma lipid profile, activity of enzymes of lipid metabolism, and aortic angiotensin converting enzyme (ACE) also studied. The overall results have proved that diosgenin attenuates CRF-induced impairment in acetylcholine induced endothelium-dependent and sodium nitroprusside induced endothelium-independent vascular relaxation. Moreover, it elevates the GSH and restores the eNOS mRNA expression level. CRF-induced dyslipidemia and ACE activity was also inhibited by diosgenin treatment. This study indicates that diosgenin have enough potential to protect vasculature against oxidative stress, dyslipidemia which in turn improves the vascular function in CRF milieu.
近年来，内皮功能障碍（ED）和过度氧化应激在心血管疾病发展中的作用得到了强调。本研究旨在评估抗氧化剂薯蓣皂苷元对慢性肾衰竭（CRF）诱导的血管功能障碍的影响。通过用含有 0.75%腺嘌呤的饮食喂养大鼠来诱导 CRF，薯蓣皂苷元经口给予（每天剂量为 40mg/kg）。在器官浴中对分离的主动脉环进行等长力测量。检查大鼠主动脉中还原型谷胱甘肽（GSH）、一氧化氮代谢物和内皮型一氧化氮合酶 mRNA 的水平。此外，还研究了血浆脂质谱、脂质代谢酶的活性以及主动脉血管紧张素转换酶（ACE）。总的结果证明，薯蓣皂苷元可减轻 CRF 引起的乙酰胆碱诱导的内皮依赖性和硝普钠诱导的内皮非依赖性血管舒张功能障碍。此外，它还可升高 GSH 并恢复 eNOS mRNA 表达水平。薯蓣皂苷元还可抑制 CRF 诱导的血脂异常和 ACE 活性。这项研究表明，薯蓣皂苷元具有足够的潜力来保护血管免受氧化应激和血脂异常的影响，从而改善 CRF 环境中的血管功能。
