Center for Nanotechnology, NASA Ames Research Center, Moffett Field, CA, 94035, USA.
Mol Neurobiol. 2013 Oct;48(2):380-5. doi: 10.1007/s12035-013-8531-6. Epub 2013 Aug 24.
The ability to rapidly detect neurotransmitter release has broad implications in the study of a variety of neurodegenerative diseases. Electrochemical detection methods using carbon nanofiber nanoelectrodes integrated into the Wireless Instantaneous Neurotransmitter Concentration Sensing System (WINCS) offer many important advantages including biocompatibility, selectivity, sensitivity, and rapid adsorption kinetics. Carbon nanofiber nanoelectrodes exhibit greater selectivity and sensitivity in the electrochemical detection of neurotransmitters compared to macroelectrodes and are able to resolve a ternary mixture of dopamine (DA), serotonin (5-HT), and ascorbic acid as well as to detect individual neurotransmitters in concentrations as low as 50 nM for DA and 100 nM for 5-HT using differential pulse voltammetry. Adsorption kinetics studies and isopropyl alcohol treatments modeled on previous studies on carbon fiber microelectrodes were conducted to investigate the analogous properties on carbon nanofiber electrodes using fast-scan cyclic voltammetry with WINCS and showed analogous results in carbon nanofiber electrodes compared with carbon fiber microelectrodes.
快速检测神经递质释放的能力在研究各种神经退行性疾病方面具有广泛的意义。使用集成到无线即时神经递质浓度感应系统 (WINCS) 的碳纤维纳米纤维纳米电极的电化学检测方法具有许多重要的优势，包括生物相容性、选择性、灵敏度和快速吸附动力学。与宏观电极相比，碳纤维纳米纤维电极在神经递质的电化学检测中表现出更高的选择性和灵敏度，并且能够分辨多巴胺 (DA)、血清素 (5-HT) 和抗坏血酸的三元混合物，并且能够使用差分脉冲伏安法以低至 50 nM 的浓度检测单个神经递质。DA 和 100 nM 的 5-HT。使用 WINCS 进行的基于先前碳纤维微电极研究的吸附动力学研究和异丙醇处理，研究了类似的特性在使用快速扫描循环伏安法的碳纤维纳米纤维电极上的特性，并与碳纤维微电极相比，在碳纤维纳米纤维电极上得到了类似的结果。
