Cope E M, Dilly S A
Department of Histopathology, St. George's Hospital Medical School, London, England.
Clin Exp Immunol. 1990 Sep;81(3):485-8. doi: 10.1111/j.1365-2249.1990.tb05360.x.
Immunohistological assessment of Kupffer cells was made using the antibody MAC387 and an antibody to lysozyme. Autopsy liver samples from 13 fetuses aged from 17 weeks gestation to term, and from 10 neonates and children aged 1 day to 18 months, were studied. For comparison, 10 normal adult autopsy liver specimens were included. The number of positively staining cells per unit area was counted for periportal sinusoids (zone 1) and centrilobular sinusoids (zone 3). No difference was found between zone 1 and zone 3 macrophage numbers with either antibody at any stage of development. Hepatic sinusoidal macrophage numbers were low during early gestation but increased during intra-uterine life to reach approximately normal adult values in the neonatal period. The numbers of cells staining with MAC387 or lysozyme were similar in each case except for hepatic sinusoidal macrophages in fetuses of less than 30 weeks gestation. Here anti-lysozyme stained significantly fewer cells, suggesting that lysozyme production may be low in immature fetuses. No difference was found between infants of similar maturity who had died immediately or had lived for more than 48 h and hence been exposed to gut antigens.
使用抗体MAC387和溶菌酶抗体对枯否细胞进行免疫组织学评估。研究了13例妊娠17周龄至足月胎儿以及10例1日龄至18个月龄新生儿和儿童的尸检肝脏样本。作为对照，纳入了10例正常成人尸检肝脏标本。对门周血窦（1区）和小叶中心血窦（3区）每单位面积的阳性染色细胞数量进行计数。在发育的任何阶段，使用任一抗体时，1区和3区巨噬细胞数量均未发现差异。肝血窦巨噬细胞数量在妊娠早期较低，但在宫内生活期间增加，在新生儿期达到近似正常成人水平。除妊娠不足30周胎儿的肝血窦巨噬细胞外，每种情况下用MAC387或溶菌酶染色的细胞数量相似。在此，抗溶菌酶染色的细胞明显较少，提示未成熟胎儿的溶菌酶产生可能较低。在相似成熟度的婴儿中，立即死亡或存活超过48小时因而接触过肠道抗原的婴儿之间未发现差异。
