Vanderwolf C H, Fine A, Cooley R K
Department of Psychology, University of Western Ontario, London, Canada.
Exp Brain Res. 1990;81(2):426-32. doi: 10.1007/BF00228136.
Unilateral injections of kainic acid into the basal forebrain in a series of rats resulted in an increase in large amplitude slow waves, a correlated burst-suppression pattern of multi-unit activity, and a decrease in acetylcholinesterase staining in the neocortex ipsilateral to the kainic acid injection. Subsequently, a cell suspension, prepared from rat embryonic basal forebrain tissue, was injected adjacent to the recording electrodes ipsilateral to the kainic acid injection. This produced a gradual recovery of low voltage fast activity (LVFA) and a correlated continuous discharge pattern of multi-unit activity in the neocortex ipsilateral to the kainic acid injection. LVFA recovered more slowly at neocortical recording sites that received an injection of a cell suspension of hippocampal primordial cells or no injection at all. Acetylcholinesterase-positive fibers from the basal forebrain tissue invaded host cortex; no comparable outrgrowths were demonstrable in the hippocampal primordium tissue grafts. Restoration of cholinergic electrocortical activation may play an important role in the improvements in behavioral performance produced by basal forebrain grafts in the cortex in animals with basal forebrain lesions.
在一系列大鼠中，向基底前脑单侧注射海藻酸，导致大鼠大脑皮层同侧出现高幅慢波增加、多单位活动相关的爆发抑制模式以及乙酰胆碱酯酶染色减少（注射海藻酸的同侧大脑皮层）。随后，将由大鼠胚胎基底前脑组织制备的细胞悬液，注射到与注射海藻酸同侧的记录电极附近。这使得低电压快活动（LVFA）逐渐恢复，并在注射海藻酸同侧的大脑皮层产生了多单位活动相关的持续放电模式。在接受海马原基细胞悬液注射或未注射的大脑皮层记录部位，LVFA恢复得更慢。来自基底前脑组织的乙酰胆碱酯酶阳性纤维侵入宿主皮层；在海马原基组织移植中未发现类似的向外生长。胆碱能电皮层激活的恢复可能在基底前脑损伤动物的皮层中，基底前脑移植所产生的行为表现改善中发挥重要作用。
