Johnston M V, McKinney M, Coyle J T
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5392-6. doi: 10.1073/pnas.76.10.5392.
Unilateral stereotaxic injection of 3.5 nmol of kainic acid into the ventral globus pallidus of rats reduced biochemical cholinergic neuronal markers by 45-50% and virtually eliminated histochemical staining for acetylcholinesterase in neocortex ipsilateral to the lesion. At the lesion site, the large, multipolar neurons that stain densely for acetylcholinesterase were absent when compared with the uninjected side. Kainate was as effective as electrocoagulation for reducing cholinergic markers although it did not affect aminergic projections ascending through the lesioned area. The conclusion that the cholinergic projection originated in neuronal perikarya at the lesion site was supported by the failure of kainate or electrolytic lesions in contiguous regions to produce similar effects. These studies provide strong evidence for a cholinergic projection to neocortex from neurons in the forebrain in the nucleus basalis.
向大鼠腹侧苍白球单侧立体定向注射3.5纳摩尔的红藻氨酸，可使生化胆碱能神经元标志物减少45 - 50%，并几乎消除损伤同侧新皮质中乙酰胆碱酯酶的组织化学染色。在损伤部位，与未注射侧相比，对乙酰胆碱酯酶染色浓密的大的多极神经元缺失。红藻氨酸在减少胆碱能标志物方面与电凝效果相同，尽管它不影响通过损伤区域上行的胺能投射。红藻氨酸或相邻区域的电解损伤未能产生类似效应，这支持了胆碱能投射起源于损伤部位神经元胞体的结论。这些研究为基底核前脑神经元向新皮质的胆碱能投射提供了有力证据。
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