Martinello Marianna, Baggio Alessandra, Gallina Albino, Mutinelli Franco
Centro di Referenza Nazionale per l'Apicoltura, Istituto Zooprofilattico Sperimentale delle Venezie , Viale dell'Università 10, 35020 Legnaro (Padova), Italy.
J Agric Food Chem. 2013 Sep 25;61(38):9275-9. doi: 10.1021/jf4027052. Epub 2013 Sep 10.
This study was performed to evaluate the distribution and depletion of sulfathiazole in different beehive matrices: honey, honeybees, "pre-existing" honeycomb, "new" honeycomb, and capping wax. Sulfathiazole was dissolved in sugar syrup or directly powdered on the combs, the matrices were sampled at different time points, and sulfathiazole residues were quantified by high-performance liquid chromatography with fluorescence detection. In honey, the higher concentration of sulfathiazole (180 mg kg(-1)) occurred 2 weeks after the last treatment in syrup. In beeswax, drug concentration was higher than in honey, particularly with powder administration, with a maximum level (340 mg kg(-1)) 3 days following the last treatment. The strongest contamination in honeybees (28 mg kg(-1)) was achieved with sulfathiazole administered in powder 3 days after the second treatment. The high persistence of sulfathiazole in the different beehive matrices suggests that it could be a reliable marker of previous treatments performed by beekeepers.
本研究旨在评估磺胺噻唑在不同蜂巢基质（蜂蜜、蜜蜂、“原有”蜂巢、“新”蜂巢和封盖蜡）中的分布及消耗情况。将磺胺噻唑溶解于糖浆中或直接撒在蜂巢上，在不同时间点对基质进行采样，并通过带荧光检测的高效液相色谱法定量磺胺噻唑残留量。在蜂蜜中，最后一次糖浆处理后2周出现了较高浓度的磺胺噻唑（180 mg kg⁻¹）。在蜂蜡中，药物浓度高于蜂蜜，尤其是采用撒粉给药时，最后一次处理后3天达到最高水平（340 mg kg⁻¹）。第二次处理后3天采用撒粉方式施用磺胺噻唑，蜜蜂体内的污染最为严重（28 mg kg⁻¹）。磺胺噻唑在不同蜂巢基质中的高持久性表明，它可能是养蜂人先前处理的可靠标志物。
