Sheth H B, Sporns P
University of Alberta, Department of Food Science, Edmonton, Canada.
J Assoc Off Anal Chem. 1990 Nov-Dec;73(6):871-4.
A simple enzyme immunoassay (EIA) was developed to screen honey samples for sulfathiazole (ST) adulteration. Honey samples required only a 30-fold dilution before use in the procedure. Because 96 well microtiter plates were used and only 100 microL of diluted honey sample was required per well, numerous replicates or samples could be tested simultaneously. The EIA was able to detect at least 0.3 ppm levels of ST in honey and also provide a rough quantitation of ST amounts.
开发了一种简单的酶免疫测定法（EIA）来筛查蜂蜜样品中是否掺有磺胺噻唑（ST）。蜂蜜样品在用于该程序之前仅需稀释30倍。由于使用了96孔微量滴定板，且每孔仅需100微升稀释后的蜂蜜样品，因此可以同时检测大量重复样品或多个样品。该酶免疫测定法能够检测蜂蜜中至少0.3 ppm水平的ST，还能对ST的含量进行大致定量。
