Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University, Nara, Japan.
Microvasc Res. 2013 Nov;90:169-72. doi: 10.1016/j.mvr.2013.08.005. Epub 2013 Aug 24.
To elucidate the pathogenic roles of oxidative stress on blood-brain-barrier (BBB) dysfunction, we compared the chronological changes of oxidative stress in blood and cerebral tissue between stroke-prone spontaneously hypertensive rats (SHRSP) and normotensive Wistar-Kyoto rats (WKY). Plasma and tissue oxidative stress was assayed by the diacron-reactive oxygen metabolite (d-ROM) test using 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a reference oxidative stress marker. The plasma and cerebral cortex d-ROM levels increased in SHRSP after 16weeks of age, but not in WKY. There were no significant differences in 8-OHdG or lipid peroxidation markers between SHRSP and WKY. Antioxidant capacity, as estimated by the biological antioxidant potential test, was similar between SHRSP and WKY at all ages examined. The changes in plasma and tissue d-ROM levels coincided with changes in glucose transporter-1 and aquaporin-4 expression, as functional constituents of the BBB. These results indicate that plasma oxidative stress increases before the onset of tissue damage, and plays an important role in BBB dysfunction rather than decreases in antioxidant capacity. The plasma d-ROM test appears to be useful for predicting vasogenic cerebral edema in severe hypertension.
为了阐明氧化应激对血脑屏障（BBB）功能障碍的致病作用，我们比较了易发生中风的自发性高血压大鼠（SHRSP）和正常血压的 Wistar-Kyoto 大鼠（WKY）之间血液和脑组织中氧化应激的时间变化。通过使用 8-羟基-2'-脱氧鸟苷（8-OHdG）作为参考氧化应激标志物，使用 Diacron 反应性氧代谢物（d-ROM）测试来测定血浆和组织中的氧化应激。在 16 周龄后，SHRSP 的血浆和大脑皮质 d-ROM 水平升高，但 WKY 没有升高。8-OHdG 或脂质过氧化标志物在 SHRSP 和 WKY 之间没有显著差异。抗氧化能力，如通过生物抗氧化潜力测试估计，在所有检查的年龄，在 SHRSP 和 WKY 之间是相似的。血浆和组织 d-ROM 水平的变化与 BBB 的功能成分葡萄糖转运蛋白-1 和水通道蛋白-4 的表达变化相吻合。这些结果表明，血浆氧化应激在组织损伤发生之前增加，并在 BBB 功能障碍中起重要作用，而不是抗氧化能力的降低。血浆 d-ROM 测试似乎可用于预测严重高血压中的血管源性脑水肿。
