Taylor M B, Easmon C S
Department of Medical Microbiology, St. Mary's Hospital Medical School, London, U.K.
FEMS Microbiol Lett. 1990 Jun 15;58(1):49-53. doi: 10.1016/0378-1097(90)90102-v.
Pneumocystis carinii pneumonia was induced in rats by chronic corticosteroid immunosuppression. The parasite was separated from virtually all contaminating host cells using the technique of unit gravity sedimentation. Cellular contamination was routinely below 0.02%. The same technique allowed partial separation of the cyst from the trophozoite stage.