Read J A, Burns S M
Regional Virus Laboratory, City Hospital, Edinburgh, UK.
Int J Exp Pathol. 1991 Dec;72(6):673-7.
Existing methods for purification of Pneumocystis carinii are unsatisfactory. Dialysis is a simple, non-stressful treatment which aided purification of organisms from rat cells. Yields were quantitated using a Petroff-Hausser chamber and nuclear staining. Following dialysis overnight, three times the yields of similar, undialysed preparations were obtained, with less than 0.2% contamination by lung cells. Overnight incubation at 4 degrees C also improved separation, but yields were only half those following dialysis. Dialysis is now used routinely in the preparation of pure Pneumocystis carinii suspensions.
现有的卡氏肺孢子虫纯化方法并不理想。透析是一种简单、无应激的处理方法，有助于从大鼠细胞中纯化生物体。使用血细胞计数板和核染色对产量进行定量。透析过夜后，获得的产量是类似的未透析制剂的三倍，肺细胞污染率低于0.2%。在4℃下过夜孵育也改善了分离效果，但产量仅为透析后的一半。现在透析常规用于制备纯卡氏肺孢子虫悬液。
