The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing University, Nanjing, 210008, P. R. China.
Analyst. 2013 Nov 7;138(21):6323-30. doi: 10.1039/c3an01255g.
A robust, nanobiotechnology-based electrochemical cytosensing platform for the detection of acute leukemia cells was developed with high sensitivity, selectivity, acceptable rapidity and excellent extensibility. It utilized the competitive binding of cell-specific aptamers to acute leukemia cells and subsequent voltammetric quantification of the metal signature. Greatly enhanced sensitivity was achieved with dual signal amplification by using Fe3O4 magnetic nanoparticles (MNPs) as carriers to load a large amount of gold nanoparticles (AuNPs) and AuNP-catalyzed silver deposition. The proposed competitive cytosensor showed high sensitivity with a detection limit down to 10 cells. This simple and low-cost electrochemical cytosensing approach offers great promise to extend its application to early detection of human leukemia and possibly to other cancer cells.
开发了一种基于纳米生物技术的稳健电化学细胞传感平台，用于高灵敏度、选择性、可接受的快速性和出色的可扩展性检测急性白血病细胞。它利用细胞特异性适体与急性白血病细胞的竞争结合，以及随后对金属标记物的伏安法定量。通过使用 Fe3O4 磁性纳米粒子 (MNPs) 作为载体来负载大量的金纳米粒子 (AuNPs) 并进行 AuNP 催化的银沉积，实现了双重信号放大，从而大大提高了灵敏度。所提出的竞争性细胞传感器具有很高的灵敏度，检测限低至 10 个细胞。这种简单且低成本的电化学生物传感器方法具有很大的应用前景，可以将其应用扩展到人类白血病的早期检测，甚至可能扩展到其他癌细胞的检测。
