Department of Clinical Laboratory, The Second Clinical Medical College, Yangtze University, Jingzhou, 434020, China.
School of Medicine, Yangtze University, Jingzhou, 434023, China.
Sci Rep. 2018 Jun 21;8(1):9444. doi: 10.1038/s41598-018-27792-5.
Hepatitis B virus (HBV) infection is a major global public health problem and one of the leading causes of chronic liver disease. HBsAg is the first serological marker to appear in the blood and is the most important marker of HBV infection. Detection of HBsAg in serum samples is commonly carried out using an immunoassay such as an enzyme-linked immunosorbent assay (ELISA), which is complex to perform, time-consuming, and unsatisfactory for testing sensitivity. Therefore, new methods for highly sensitive detection of HBV infection are urgently needed. Aptamers are specific recognition molecules with high affinity and specificity toward their targets. Biosensors that employ aptamers as biorecognition elements are known as aptasensors. In this study, we select an HBsAg-specific aptamer and use it to develop a new chemiluminescent aptasensor based on rapid magnetic separation and double-functionalized gold nanoparticles. This sensor enables rapid magnetic separation and highly sensitive detection of HBsAg in HBV-positive serum. The detection limit of this HBsAg-detecting chemiluminescent aptasensor is as low as 0.05 ng/mL, which is much lower than the 0.5 ng/mL limit of a typical ELISA used in hospitals. Furthermore, this aptasensor works well and is highly specific to HBV infection.
乙型肝炎病毒 (HBV) 感染是一个全球性的重大公共卫生问题，也是导致慢性肝病的主要原因之一。HBsAg 是最早出现在血液中的血清学标志物，也是 HBV 感染最重要的标志物。通常使用酶联免疫吸附试验 (ELISA) 等免疫测定法检测血清样本中的 HBsAg，这种方法操作复杂、耗时且检测灵敏度不理想。因此，迫切需要新的方法来高度敏感地检测 HBV 感染。适体是对其靶标具有高亲和力和特异性的特异性识别分子。以适体作为生物识别元件的生物传感器称为适体传感器。在本研究中，我们选择了一种 HBsAg 特异性适体，并利用它开发了一种基于快速磁分离和双功能化金纳米粒子的新型化学发光适体传感器，用于检测 HBV 阳性血清中的 HBsAg。该传感器能够实现快速磁分离和对 HBsAg 的高灵敏度检测，其检测限低至 0.05ng/mL，远低于医院常用的典型 ELISA 的 0.5ng/mL 检测限。此外，该适体传感器性能良好，对 HBV 感染具有高度特异性。
