喹诺酮类药物耐药蛋白 QnrB1 的突变分析。
Mutational analysis of quinolone resistance protein QnrB1.
机构信息
Lahey Hospital and Medical Center, Burlington, Massachusetts, USA.
出版信息
Antimicrob Agents Chemother. 2013 Nov;57(11):5733-6. doi: 10.1128/AAC.01533-13. Epub 2013 Aug 26.
Abstract
Alanine substitutions and selected deletions have been used to localize amino acids in QnrB essential for its protective activity. Essential amino acids are found at positions i and i(-2) in the pentapeptide repeat module and in the larger of two loops, where deletion of only a single amino acid compromises activity. Deletion of 10 amino acids at the N terminus is tolerated, but removal of 3 amino acids in the C-terminal dimerization unit destroys activity.
摘要
丙氨酸取代和选择性缺失已被用于定位 QnrB 中对其保护活性至关重要的氨基酸。必需氨基酸位于五肽重复模块的 i 位和 i(-2)位以及两个环中的大环中，其中仅缺失一个氨基酸就会影响活性。N 端缺失 10 个氨基酸是可以耐受的，但 C 端二聚化单元中去除 3 个氨基酸会破坏活性。
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