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Intra- and Interlaboratory Agreement in Assessing the In Vitro Activity of Micafungin against Common and Rare Candida Species with the EUCAST, CLSI, and Etest Methods.采用EUCAST、CLSI和Etest方法评估米卡芬净对常见和罕见念珠菌体外活性的实验室内部和实验室间一致性
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本文引用的文献

1
Increasing echinocandin resistance in Candida glabrata: clinical failure correlates with presence of FKS mutations and elevated minimum inhibitory concentrations.棘白菌素类耐药性在光滑念珠菌中的增加：临床失败与 FKS 突变和最低抑菌浓度升高相关。
Clin Infect Dis. 2013 Jun;56(12):1724-32. doi: 10.1093/cid/cit136. Epub 2013 Mar 13.
2
The presence of an FKS mutation rather than MIC is an independent risk factor for failure of echinocandin therapy among patients with invasive candidiasis due to Candida glabrata.棘白菌素治疗失败的独立危险因素是侵袭性念珠菌病（由光滑念珠菌引起）患者存在 FKS 突变而不是 MIC。
Antimicrob Agents Chemother. 2012 Sep;56(9):4862-9. doi: 10.1128/AAC.00027-12. Epub 2012 Jul 2.
3
Progress in antifungal susceptibility testing of Candida spp. by use of Clinical and Laboratory Standards Institute broth microdilution methods, 2010 to 2012.2010 至 2012 年，利用临床和实验室标准协会微量肉汤稀释法对抗真菌药敏试验中念珠菌属的研究进展。
J Clin Microbiol. 2012 Sep;50(9):2846-56. doi: 10.1128/JCM.00937-12. Epub 2012 Jun 27.
4
EUCAST technical note on the EUCAST definitive document EDef 7.2: method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for yeasts EDef 7.2 (EUCAST-AFST).EUCAST 技术说明：EUCAST 确诊文件 EDef 7.2：抗真菌药物检测酵母菌肉汤稀释最低抑菌浓度的方法（EUCAST-AFST）。
Clin Microbiol Infect. 2012 Jul;18(7):E246-7. doi: 10.1111/j.1469-0691.2012.03880.x. Epub 2012 May 8.
5
Recurrent episodes of candidemia due to Candida glabrata with a mutation in hot spot 1 of the FKS2 gene developed after prolonged therapy with caspofungin.由于 FKS2 基因热点 1 突变的光滑念珠菌导致的念珠菌血症反复发作，在长期使用卡泊芬净治疗后发生。
Antimicrob Agents Chemother. 2012 Jun;56(6):3417-9. doi: 10.1128/AAC.06100-11. Epub 2012 Mar 5.
6
Echinocandin susceptibility testing of Candida isolates collected during a 1-year period in Sweden.在瑞典进行的为期一年的研究中，对收集到的念珠菌分离株进行棘白菌素药敏试验。
J Clin Microbiol. 2011 Jul;49(7):2516-21. doi: 10.1128/JCM.00201-11. Epub 2011 May 4.
7
Disseminated Candidiasis caused by Candida albicans with amino acid substitutions in Fks1 at position Ser645 cannot be successfully treated with micafungin.氟康唑耐药白念珠菌 Fks1 第 645 位丝氨酸突变为氨基酸替换型导致的播散性念珠菌病，米卡芬净治疗无效。
Antimicrob Agents Chemother. 2011 Jul;55(7):3075-83. doi: 10.1128/AAC.01686-10. Epub 2011 Apr 18.
8
Comparison of European Committee on Antimicrobial Susceptibility Testing (EUCAST) and Etest methods with the CLSI broth microdilution method for echinocandin susceptibility testing of Candida species.比较欧洲抗菌药物敏感性试验委员会 (EUCAST) 和 Etest 方法与 CLSI 肉汤微量稀释法检测念珠菌属对棘白菌素类药物敏感性。
J Clin Microbiol. 2010 May;48(5):1592-9. doi: 10.1128/JCM.02445-09. Epub 2010 Mar 24.
9
Echinocandin susceptibility testing of Candida species: comparison of EUCAST EDef 7.1, CLSI M27-A3, Etest, disk diffusion, and agar dilution methods with RPMI and isosensitest media.棘白菌素类药物敏感性检测方法：比较 EUCAST EDef 7.1、CLSI M27-A3、Etest、纸片扩散法和琼脂稀释法与 RPMI 和 IsoSensitest 培养基的结果。
Antimicrob Agents Chemother. 2010 Jan;54(1):426-39. doi: 10.1128/AAC.01256-09. Epub 2009 Nov 2.
10
Comparison of antifungal MICs for yeasts obtained using the EUCAST method in a reference laboratory and the Etest in nine different hospital laboratories.参考实验室采用 EUCAST 方法和 9 家不同医院实验室的 Etest 法检测酵母菌抗真菌 MIC 值的比较。
Clin Microbiol Infect. 2010 Jul;16(7):863-9. doi: 10.1111/j.1469-0691.2009.02997.x. Epub 2009 Sep 23.

EUCAST 程序与 Etest 测定分离的念珠菌属对米卡芬净敏感性的比较。

Comparison between the EUCAST procedure and the Etest for determination of the susceptibility of Candida species isolates to micafungin.

机构信息

Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain.

出版信息

Antimicrob Agents Chemother. 2013 Nov;57(11):5767-70. doi: 10.1128/AAC.01032-13. Epub 2013 Aug 26.

DOI:10.1128/AAC.01032-13
PMID:23979756
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3811256/
Abstract

We compared the ability of the EUCAST EDef 7.2 and the Etest to detect the susceptibility to micafungin of 160 Candida and non-Candida clinical isolates. Agreement was higher when Etest MICs were obtained after 24 h of incubation; essential agreement was 90%, and categorical agreement was >90%. False susceptibility was seen only for Candida krusei (10%), and false resistance was observed in 6% of the isolates, ranging from 2.6% (C. glabrata) to 13% (C. albicans).

摘要

我们比较了 EUCAST EDef 7.2 和 Etest 检测 160 株念珠菌和非念珠菌临床分离株对米卡芬净敏感性的能力。孵育 24 小时后获得的 Etest MIC 时，一致性更高；基本一致率为 90%，分类一致率>90%。仅对克柔念珠菌（10%）出现假敏感性，6%的分离株出现假耐药性，范围为 2.6%（近平滑念珠菌）至 13%（白色念珠菌）。