Laboratory of Enzyme Engineering and Microbiology, Ecole Nationale d'Ingénieurs de Sfax, University of Sfax, Route de Soukra km 4.5, BP 1173, 3038, Sfax, Tunisia.
Laboratory of Soil Biology, University of Neuchatel, Rue Emile Argand 11, 2009, Neuchatel, Switzerland.
Environ Sci Pollut Res Int. 2014 Feb;21(3):1746-1758. doi: 10.1007/s11356-013-2019-9. Epub 2013 Aug 25.
The search for novel microorganisms able to degrade olive mill wastewaters (OMW) and withstand the toxic effects of the initially high phenolic concentrations is of great scientific and industrial interest. In this work, the possibility of reducing the phenolic content of OMW using new isolates of fungal strains (Coriolopsis gallica, Bjerkandera adusta, Trametes versicolor, Trichoderma citrinoviride, Phanerochaete chrysosporium, Gloeophyllum trabeum, Trametes trogii, and Fusarium solani) was investigated. In vitro, all fungal isolates tested caused an outstanding decolorization of OMW. However, C. gallica gave the highest decolorization and dephenolization rates at 30 % v/v OMW dilution in water. Fungal growth in OMW medium was affected by several parameters including phenolic compound concentration, nitrogen source, and inoculum size. The optimal OMW medium for the removal of phenolics and color was with the OMW concentration (in percent)/[(NH4)2SO4]/inoculum ratio of 30:6:3. Under these conditions, 90 and 85 % of the initial phenolic compounds and color were removed, respectively. High-pressure liquid chromatography analysis of extracts from treated and untreated OMW showed a clear and substantial reduction in phenolic compound concentrations. Phytotoxicity, assessed using radish (Raphanus sativus) seeds, indicated an increase in germination index of 23-92 % when a 30 % OMW concentration was treated with C. gallica in different dilutions (1/2, 1/4, and 1/8).
寻找能够降解橄榄厂废水 (OMW) 并耐受最初高酚浓度的毒性影响的新型微生物具有重要的科学和工业意义。在这项工作中，研究了使用新分离的真菌菌株（Coriolopsis gallica、Bjerkandera adusta、Trametes versicolor、Trichoderma citrinoviride、Phanerochaete chrysosporium、Gloeophyllum trabeum、Trametes trogii 和 Fusarium solani）降低 OMW 中酚含量的可能性。在体外，所有测试的真菌分离物都导致 OMW 出色的脱色。然而，在 30%v/v OMW 稀释水中，C. gallica 给出了最高的脱色和脱酚率。真菌在 OMW 培养基中的生长受到多种参数的影响，包括酚类化合物浓度、氮源和接种量。去除酚类和颜色的最佳 OMW 培养基是 OMW 浓度（%）/[(NH4)2SO4]/接种量比为 30:6:3。在这些条件下，初始酚类化合物和颜色分别去除了 90%和 85%。用处理和未处理的 OMW 提取物进行高效液相色谱分析表明，酚类化合物浓度明显降低。用萝卜（Raphanus sativus）种子评估的植物毒性表明，当用不同稀释度（1/2、1/4 和 1/8）的 C. gallica 处理 30%的 OMW 时，发芽指数增加了 23-92%。
