Democritus University of Thrace, Department of Environmental Engineering, Laboratory of Wastewater Management and Treatment Technologies, Vas. Sofias 12, Xanthi, Greece.
Chemosphere. 2012 Jul;88(5):620-6. doi: 10.1016/j.chemosphere.2012.03.042. Epub 2012 Apr 3.
Thirty-nine white-rot fungi belonging to nine species of Agaricomycotina (Basidiomycota) were initially screened for their ability to decrease olive-mill wastewater (OMW) phenolics. Four strains of Ganoderma australe, Ganoderma carnosum, Pleurotus eryngii and Pleurotus ostreatus, were selected and further examined for key-aspects of the OMW biodegradation process. Fungal growth in OMW-containing batch cultures resulted in significant decolorization (by 40-46% and 60-65% for Ganoderma and Pleurotus spp. respectively) and reduction of phenolics (by 64-67% and 74-81% for Ganoderma and Pleurotus spp. respectively). COD decrease was less pronounced (12-29%). Cress-seeds germination increased by 30-40% when OMW was treated by Pleurotus strains. Toxicity expressed as inhibition of Aliivibrio fischeri luminescence was reduced in fungal-treated OMW samples by approximately 5-15 times compared to the control. As regards the pertinent enzyme activities, laccase and Mn-independent peroxidase were detected for Ganoderma spp. during the entire incubation period. In contrast, Pleurotus spp. did not exhibit any enzyme activities at early growth stages; instead, high laccase (five times greater than those of Ganoderma spp.) and Mn peroxidases activities were determined at the end of treatment. OMW decolorization by Ganoderma strains was strongly correlated to the reduction of phenolics, whereas P. eryngii laccase activity was correlated with the effluent's decolorization.
最初筛选了 39 种属于 Agaricomycotina（担子菌门）的 9 个种的白腐真菌，以评估它们降低橄榄厂废水（OMW）中酚类物质的能力。选择了 4 株灵芝、红肉灵芝、杏鲍菇和糙皮侧耳，并进一步研究了它们对 OMW 生物降解过程的关键方面。真菌在含 OMW 的分批培养中的生长导致明显的脱色（灵芝和糙皮侧耳分别为 40-46%和 60-65%）和酚类物质的减少（灵芝和糙皮侧耳分别为 64-67%和 74-81%）。COD 减少量不明显（12-29%）。当 OMW 被糙皮侧耳菌株处理时，水芹种子的发芽率增加了 30-40%。与对照相比，真菌处理后的 OMW 样品中，以抑制发光菌 Aliivibrio fischeri 发光表示的毒性降低了约 5-15 倍。就相关酶活性而言，灵芝属在整个培养期间均检测到漆酶和 Mn 非依赖性过氧化物酶。相比之下，糙皮侧耳在早期生长阶段没有表现出任何酶活性；相反，在处理结束时测定了高漆酶（比灵芝属高 5 倍）和 Mn 过氧化物酶活性。灵芝菌株对 OMW 的脱色与酚类物质的减少密切相关，而 P. eryngii 漆酶活性与废水的脱色相关。
