[白芍总苷对D-半乳糖致衰大鼠脑组织Toll受体及白细胞介素-33 mRNA表达的影响：一项实验研究]

[Effects of total paeony glucosides on mRNA expressions of Toll receptors and interleukin-33 in the brain tissue of D-galactose induced aging rats: an experimental research].

作者信息

Zhang Hai-Yan, Liu Zhong-Jin, Chen Zhi-Wei

机构信息

Department of Histology and Embryology, Qiqihar Medical College, Heilongjiang 161006, China.

出版信息

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2013 Jun;33(6):830-3.

PMID:23980368

Abstract

OBJECTIVE

To investigate effects of total paeony glucosides (TPGs) on the expressions of Toll receptors (TLR4) and interleukin-33 (IL-33) in the brain tissue of D-galactose-induced aging rats. METHODS; Fifty SD rats were randomly divided into 5 groups, i.e., the blank control group, the model group, the high dose TPG group, the middle dose TPG group, and the low dose TPG group, 10 in each group. Equal volume of normal saline was subcutaneously injected to rats in the blank control group, while 10% D-galactose was subcutaneously injected to rats in the rest groups at 0.125 mL/g, once a day for 8 successive weeks to induce the aging rat model. TPG was administered at 300 mg/kg, 150 mg/kg, and 75 mg/kg to rats in the high, middle, and low dose TPG groups while injecting D-galactose from the 5th week of model preparation, once daily for 4 successive weeks. Equal volume of normal saline was administered to rats in the blank control group and the model group, once daily. The capability for learning and memory was detected using Morris water. The mRNA expressions of TLR4 and IL-33 in the brain tissue were detected using ELISA.

RESULTS

Compared with the blank control group, the capability for learning and memory decreased in the model group with statistical difference (P < 0.05). Compared with the model group, the capability for learning and memory was obviously improved in all the medicated groups in a dose-dependent manner, showing statistical difference (P < 0.05). Compared with the blank control group, mRNA expressions of TLR4 and IL-33 in the brain tissue obviously increased after medication in the model group, showing statistical difference (P < 0.05). Compared with the model group, mRNA expressions of TLR4 and IL-33 in the brain tissue obviously decreased after medication in all the medicated groups in a dose-dependent manner, showing statistical difference (P < 0.05).

CONCLUSION

TPGs improved D-galactose induced aging rats' capability for learning and memory through regulating changes of TLR4 and IL-33 expressions.

摘要

目的

探讨白芍总苷（TPGs）对D-半乳糖致衰老大鼠脑组织中Toll样受体4（TLR4）和白细胞介素-33（IL-33）表达的影响。方法：将50只SD大鼠随机分为5组，即空白对照组、模型组、TPG高剂量组、TPG中剂量组和TPG低剂量组，每组10只。空白对照组大鼠皮下注射等体积生理盐水，其余各组大鼠皮下注射10% D-半乳糖，剂量为0.125 mL/g，每天1次，连续8周，诱导衰老大鼠模型。从造模第5周起，TPG高、中、低剂量组大鼠分别按300 mg/kg、150 mg/kg、75 mg/kg给药，连续4周，每天1次。空白对照组和模型组大鼠给予等体积生理盐水，每天1次。采用Morris水迷宫检测学习记忆能力。采用ELISA法检测脑组织中TLR4和IL-33的mRNA表达。结果：与空白对照组比较，模型组大鼠学习记忆能力下降，差异有统计学意义（P < 0.05）。与模型组比较，各用药组大鼠学习记忆能力均明显提高，且呈剂量依赖性，差异有统计学意义（P < 0.05）。与空白对照组比较，模型组用药后脑组织中TLR4和IL-33的mRNA表达明显升高，差异有统计学意义（P < 0.05）。与模型组比较，各用药组用药后脑组织中TLR4和IL-33的mRNA表达均明显降低，且呈剂量依赖性，差异有统计学意义（P < 0.05）。结论：TPGs通过调节TLR4和IL-33表达的变化改善D-半乳糖致衰老大鼠的学习记忆能力。