[五鹤续断对D-半乳糖致小鼠衰老模型的影响]

[The effect of Wu-He Dipsacus asper on mice-aging model induced by D-galactose].

作者信息

Zhan Guang-Jie, Yang Nian-An, Xiao Ben-Jian

出版信息

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2014 Mar;30(2):174-7.

Abstract

OBJECTIVE

To study the effect of Wu-He Dipsacus asper (WHDA), Traditional Chinese Medicine, injection on mice-aging model induced by D-galactose.

METHODS

Forty-eight Kunming mice (24 male and 24 female) were randomly divided into control group, model group, positive control group, 7.2 g/kg WHDA group, 3.6 g/kg WHDA group and 1.8 g/kg WHDA group with eight in each group. The model was induced through injecting D-galactose into peritoneal cavity and Morris water maze was used to detect the learning and cognitive ability of mice. The skin hydroxyproline, brain tissue malondialdehyde (MDA), lipofuscin (LP), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels of mice were detected; the IL-2 and IL-6 levels in serum of mice were detected by using double antibody sandwich ELISA method.

RESULTS

Each WHDA group was significantly reduced in latency period compared with the model group during Morris water maze test (P < 0.05) and the number of mice in model group through the platform was less than other mice in each group (P < 0.05). The levels of MAD and LP of the control group and each WHDA group were less than model group in the detection of heart, brain tissue oxidation index (SOD, MAD, LP and GSH-Px, P < 0.05). The activity of SOD and GSH-Px in the control group and each WHDA group was significantly higher than that in the model group (P < 0.05). The skin hydroxyproline content of mice which had been injected with D-galactose was significantly lower than that in the control group (P < 0.05) and the skin hydroxyproline content of mice of WHDA group was significantly higher than that in the model group (P < 0.05). The IL-2, IL-6 levels in serum of mice in WHDA group were significantly higher than those in the control group and the model group (P < 0.05) and the IL-2, IL-6 levels in serum of mice in the model group were lower than those in the control group (P < 0.05).

CONCLUSION

The effective constituents of WHDA have a variety of biological activity which can have a good effect on anti-aging by different ways, improving learning and memory function, eliminating free radicals antioxidant, and enhancing the body immunity and other aspects.

摘要

目的

研究中药五合续断注射液对D-半乳糖诱导的小鼠衰老模型的影响。

方法

将48只昆明小鼠（雄性24只，雌性24只）随机分为对照组、模型组、阳性对照组、五合续断注射液7.2 g/kg组、3.6 g/kg组和1.8 g/kg组，每组8只。通过腹腔注射D-半乳糖诱导建立模型，采用Morris水迷宫检测小鼠的学习和认知能力。检测小鼠皮肤羟脯氨酸、脑组织丙二醛（MDA）、脂褐素（LP）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）水平；采用双抗体夹心ELISA法检测小鼠血清中IL-2和IL-6水平。

结果

在Morris水迷宫试验中，各五合续断注射液组潜伏期较模型组均显著缩短（P<0.05），模型组通过平台的小鼠数量少于其他各组（P<0.05）。检测心脏、脑组织氧化指标（SOD、MDA、LP和GSH-Px）时，对照组和各五合续断注射液组的MDA和LP水平均低于模型组（P<0.05）。对照组和各五合续断注射液组的SOD和GSH-Px活性均显著高于模型组（P<0.05）。注射D-半乳糖的小鼠皮肤羟脯氨酸含量显著低于对照组（P<0.05），五合续断注射液组小鼠皮肤羟脯氨酸含量显著高于模型组（P<0.05）。五合续断注射液组小鼠血清中IL-2、IL-6水平显著高于对照组和模型组（P<0.05），模型组小鼠血清中IL-2、IL-6水平低于对照组（P<0.05）。