[柚皮素对阿尔茨海默病模型大鼠学习记忆能力的影响]

[Effect of naringenin on learning and memory ability on model rats with Alzheimer disease].

作者信息

Ma Jing, Yang Wen-Qing, Zha He, Yu Hua-Rong

机构信息

Chongqing Medical University, Chongqing 400016, China.

出版信息

Zhong Yao Cai. 2013 Feb;36(2):271-6.

PMID:23901657

Abstract

OBJECTIVE

To investigate the effects of naringenin on the learning and memory ability of Alzheimer disease (AD) rats.

METHODS

30 male SD rats were randomly divided into control group (sham operation group), model group and naringenin group. AD model was established by injecting strepoztocin (3 mg/kg) twice into each of two intracerebroventriculas. Naringenin group were given intragastric administration of naringenin once a day for 3 weeks and the other two groups were given intragadtric administration of normal saline with the same dosage and time period. After 3 weeks, learning and memory ability in all the three groups were analyzed by Morris water maze, the activity of superoxide dismutase(SOD) and the content of malondialdehyde (MDA) of the rats' brain tissue was detected by chemical colorimetric determination. Observed the expressions of Abeta42 and Abeta40 by immunohistochemical method. The expression and degree of phosphorylation of tau protein was assayed by western blotting.

RESULTS

Compared with the sham operation group, the mean escape latency of the model group was significantly prolonged (P < 0.05) and the time that rats were in the platform quadrant was significantly shortened (P < 0.0.5). On the contrary, compared with the model group, the mean escape latency of naringenin group was significantly shortened (P < 0.05) and the time that rats were in the platform quadrant was significantly extended (P < 0.005). 2. The level of MDA in the model group, compared with the sham operation group group, was significantly increased (P < 0.05). Whereas, that of naringenin group, compared with the model group, was significantly decreased compared with the sham operation group (P < 0.05). The activity of SOD in the naringenin group was significantly increased comparing with the model group (P < 0.05). 3. The expressions of Abeta40 and Abeta42 in model group were obviously up-regulated. Instead, the expressions of Abeta40 and Abeta42 in the naringenin group were significantly down regulated. 4. There was no significant difference in the expression of tau protein among each groups. Nevertheless, the phosphorylation of tau protein in the model group was significantly enhanced than that in the control group (P < 0.05), and the phosphorylation of tau protein in the naringenin group was significantly reduced than that in the model group (P < 0.05).

CONCLUSION

Naringenin can improve learning and memory ability of model rats with Alzheimer disease through the approach of oxidative stress.

摘要

目的

探讨柚皮素对阿尔茨海默病（AD）大鼠学习记忆能力的影响。

方法

将30只雄性SD大鼠随机分为对照组（假手术组）、模型组和柚皮素组。通过向双侧脑室内注射链脲佐菌素（3 mg/kg）建立AD模型。柚皮素组大鼠每天灌胃给予柚皮素，连续3周，另外两组给予相同剂量和时间段的生理盐水灌胃。3周后，通过Morris水迷宫分析三组大鼠的学习记忆能力，采用化学比色法检测大鼠脑组织中超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量。采用免疫组织化学方法观察β淀粉样蛋白42（Aβ42）和β淀粉样蛋白40（Aβ40）的表达。通过蛋白质免疫印迹法检测tau蛋白的表达及磷酸化程度。

结果

1.与假手术组相比，模型组平均逃避潜伏期显著延长（P<0.05），大鼠在平台象限停留时间显著缩短（P<0.05）。相反，与模型组相比，柚皮素组平均逃避潜伏期显著缩短（P<0.05），大鼠在平台象限停留时间显著延长（P<0.005）。2.与假手术组相比，模型组MDA水平显著升高（P<0.05）。而与模型组相比，柚皮素组MDA水平显著降低（P<0.05）。与模型组相比，柚皮素组SOD活性显著升高（P<0.05）。3.模型组Aβ40和Aβ42表达明显上调。相反，柚皮素组Aβ40和Aβ42表达显著下调。4.各组tau蛋白表达无显著差异。然而，模型组tau蛋白磷酸化水平显著高于对照组（P<0.05），柚皮素组tau蛋白磷酸化水平显著低于模型组（P<0.05）。