Shimada Y, Sugihara A, Iizumi T, Tominaga Y
Osaka Municipal Technical Research Institute.
J Biochem. 1990 May;107(5):703-7. doi: 10.1093/oxfordjournals.jbchem.a123112.
Geotrichum candidum produces two extracellular lipases, I and II. A lipase II cDNA clone was isolated from a cDNA library by colony hybridization using the 32P-labeled fragment of lipase I cDNA isolated previously. The nucleotide sequence of lipase II cDNA determined by the dideoxy chain terminating method includes the N- and C-terminal amino acid sequences of lipase II, and the overall amino acid composition deduced from the cDNA coincides with that deduced on amino acid analysis of this protein. The cloned lipase II cDNA codes a protein of 544 amino acids and a part of the signal sequence of 13 amino acids. The peptide chain lengths of lipases I and II are the same, their overall identity being 84%. Furthermore, four Cys residues are completely conserved, which may participate in the formation of disulfide bridges. A homology search indicated that the G. candidum lipases and Candida cyclindracea lipase are homologous enzymes and that they are members of the cholinesterase family.
白地霉可产生两种细胞外脂肪酶，即脂肪酶I和脂肪酶II。利用先前分离得到的脂肪酶I cDNA的32P标记片段，通过菌落杂交从cDNA文库中分离出脂肪酶II cDNA克隆。采用双脱氧链终止法测定的脂肪酶II cDNA的核苷酸序列包含脂肪酶II的N端和C端氨基酸序列，从该cDNA推导的整体氨基酸组成与对该蛋白质进行氨基酸分析所推导的结果一致。克隆的脂肪酶II cDNA编码一个由544个氨基酸组成的蛋白质以及一段由13个氨基酸组成的信号序列。脂肪酶I和脂肪酶II的肽链长度相同，它们的整体一致性为84%。此外，四个半胱氨酸残基完全保守，可能参与二硫键的形成。同源性搜索表明，白地霉脂肪酶和圆柱假丝酵母脂肪酶是同源酶，它们属于胆碱酯酶家族。
