Lotti M, Grandori R, Fusetti F, Longhi S, Brocca S, Tramontano A, Alberghina L
Dipartimento di Fisiologia e Biochemica Generali, Università degli Studi di Milano, Italy.
Gene. 1993 Feb 14;124(1):45-55. doi: 10.1016/0378-1119(93)90760-z.
Lipases (Lip) hydrolyze triglycerides into fatty acids and glycerol. Lip produced by the yeast Candida cylindracea are encoded by multiple genomic sequences. We report the molecular cloning and characterization of three genes from this family. They encode putative mature 57-kDa proteins of 534 amino acids (aa). To date, five Lip-encoding genomic sequences from C. cylindracea have been characterized in our laboratory. The five deduced aa sequences share an overall homology of 80%. These sequences have been aligned with each other and with those of homologous enzymes, the Lip from the mould Geotrichum candidum and the acetylcholinesterase from Torpedo californica, whose three-dimensional structures have been solved by X-ray analysis. The C. cylindracea Lip appear to have a structural organization similar to that described for both enzymes.
脂肪酶（Lip）将甘油三酯水解为脂肪酸和甘油。圆柱假丝酵母产生的脂肪酶由多个基因组序列编码。我们报道了该家族三个基因的分子克隆及特性分析。它们编码推定的成熟蛋白，为含534个氨基酸（aa）的57 kDa蛋白。迄今为止，我们实验室已对圆柱假丝酵母的5个脂肪酶编码基因组序列进行了特性分析。这5个推导的氨基酸序列总体同源性为80%。这些序列已相互比对，并与同源酶（即来自白地霉的脂肪酶和来自加州电鳐的乙酰胆碱酯酶）的序列进行了比对，后者的三维结构已通过X射线分析得以解析。圆柱假丝酵母脂肪酶的结构组织似乎与上述两种酶的相似。
