几种念珠菌属物种中亮氨酸密码子CUG的非通用解码。
Non-universal decoding of the leucine codon CUG in several Candida species.
作者信息
Ohama T, Suzuki T, Mori M, Osawa S, Ueda T, Watanabe K, Nakase T
机构信息
Department of Biology, School of Science, Nagoya University, Japan.
出版信息
Nucleic Acids Res. 1993 Aug 25;21(17):4039-45. doi: 10.1093/nar/21.17.4039.
Abstract
It has been reported that CUG, a universal leucine codon, is read as serine in an asporogenic yeast, Candida cylindracea. The distribution of this non-universal genetic code in various yeast species was studied using an in vitro translation assay system with a synthetic messenger RNA containing CUG codons in-frame. It was found that CUG is used as a serine codon in six out of the fourteen species examined, while it is used for leucine in the remaining eight. The tRNA species responsible for the translation of codon CUG as serine was detected in all the six species in which CUG is translated as serine. The grouping according to the CUG codon assignments in these yeast species shows a good correlation with physiological classification by the chain lengths of the isoprenoid moiety of ubiquinone and the cell-wall sugar contained in the yeasts. The six Candida species examined in which CUG is used as serine belong to one distinct group in Hemiascomycetes.
摘要
据报道，通用的亮氨酸密码子CUG在产孢酵母圆柱假丝酵母中被解读为丝氨酸。使用含有框内CUG密码子的合成信使RNA的体外翻译测定系统，研究了这种非通用遗传密码在各种酵母物种中的分布。结果发现，在所检测的14个物种中，有6个物种将CUG用作丝氨酸密码子，而其余8个物种则将其用于亮氨酸。在所有将CUG翻译为丝氨酸的6个物种中，都检测到了负责将密码子CUG翻译为丝氨酸的tRNA种类。根据这些酵母物种中CUG密码子的分配进行的分组，与酵母中泛醌类异戊二烯部分的链长和细胞壁糖的生理分类显示出良好的相关性。所检测的6个将CUG用作丝氨酸的假丝酵母物种属于半子囊菌纲中的一个独特类群。
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