State Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China; Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, People's Republic of China.
Biotechnol Appl Biochem. 2013 Nov-Dec;60(6):623-8. doi: 10.1002/bab.1110. Epub 2013 Aug 23.
Acidithiobacillus ferrooxidans plays an important role in bioleaching in reproducing the mineral oxidant of ferric iron (Fe(3+) ) by oxidization of ferrous iron (Fe(2+) ). The high-molecular-weight c-type cytochrome Cyc2 that is located in the external membrane is postulated as the first electron carrier in the Fe(2+) oxidation respiratory pathway of A. ferrooxidans. To increase ferrous iron oxidation activity, a recombinant plasmid pTCYC2 containing cyc2 gene under the control of Ptac promoter was constructed and transferred into A. ferrooxidans ATCC19859. The transcriptional level of cyc2 gene was increased by 2.63-fold and Cyc2 protein expression was observed in the recombinant strain compared with the control. The ferrous iron oxidation activity and the arsenic stressed cell growth of the recombinant strain were also elevated.
氧化亚铁硫杆菌在生物浸矿中起着重要作用，通过氧化二价铁（Fe（2+））来再生矿物氧化剂三价铁（Fe（3+））。位于外膜上的高分子量 c 型细胞色素 Cyc2 被假定为氧化亚铁硫杆菌 Fe（2+）氧化呼吸途径中的第一个电子载体。为了提高二价铁氧化活性，构建了一个含有 Ptac 启动子控制的 cyc2 基因的重组质粒 pTCYC2，并将其转移到氧化亚铁硫杆菌 ATCC19859 中。与对照相比，cyc2 基因的转录水平提高了 2.63 倍，并且在重组菌中观察到 Cyc2 蛋白的表达。重组菌的二价铁氧化活性和砷胁迫细胞生长也得到了提高。
