Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
Biochemistry (Mosc). 2013 Jun;78(6):651-9. doi: 10.1134/S0006297913060102.
In this study, proteins specifically interacting with the 3' untranslated region (UTR) of mRNA of the multifunctional Y-box-binding protein 1 (YB-1) were identified. One of these, hnRNP Q, was shown to specifically interact with the regulatory element (RE) in YB-1 mRNA 3' UTR and to inhibit translation of this mRNA. Its binding to the RE was accompanied by displacement from this element of the poly(A)-binding protein (PABP), a positive regulator of YB-1 mRNA translation, and by enhanced binding of the negative YB-1 mRNA translation regulator - YB-1 itself.
在这项研究中，鉴定出与多功能 Y 盒结合蛋白 1（YB-1）mRNA 的 3'非翻译区（UTR）特异性相互作用的蛋白质。其中之一是 hnRNP Q，它被证明与 YB-1 mRNA 3'UTR 中的调节元件（RE）特异性相互作用，并抑制该 mRNA 的翻译。它与 RE 的结合伴随着多聚（A）结合蛋白（PABP）从该元件的位移，PABP 是 YB-1 mRNA 翻译的正调节剂，并且与负 YB-1 mRNA 翻译调节剂 YB-1 本身的结合增强。
