Institute of Protein Research, Russian Academy of Sciences, Pushchino, Russia.
RNA Biol. 2021 Nov;18(11):1630-1641. doi: 10.1080/15476286.2020.1859243. Epub 2020 Dec 27.
Y-box binding proteins are members of the family of proteins containing the evolutionarily conserved cold shock domain. Their cellular functions are quite diverse, including transcription and translation regulation, participation in pre-mRNA splicing, mRNA stabilization and packaging into mRNPs, involvement in DNA repair, and some others. To date, we know little about the plausible functional interchangeability of Y-box binding proteins. Our previous finding was that in YB-1-null HEK293T cells the synthesis of YB-3 is enhanced, thus enabling YB-3 to interact with a larger set of mRNAs and compensate for the YB-1 absence. We suggested the existence of a mechanism of YB-3 synthesis regulation by its paralog, YB-1. Here we demonstrate that YB-1 participates in the translational control and stabilization of mRNA through untranslated regions of mRNA.
Y 盒结合蛋白是含有进化上保守的冷休克结构域的蛋白家族的成员。它们的细胞功能非常多样化，包括转录和翻译调控、参与前体 mRNA 的剪接、mRNA 的稳定和包装成 mRNPs、参与 DNA 修复等。迄今为止，我们对 Y 盒结合蛋白的功能可替代性知之甚少。我们之前的发现是，在 YB-1 缺失的 HEK293T 细胞中，YB-3 的合成增强，从而使 YB-3 能够与更大的一组 mRNA 相互作用，并弥补 YB-1 的缺失。我们提出了 YB-3 合成受其同源物 YB-1 调节的机制。在这里，我们证明 YB-1 通过 mRNA 的非翻译区参与翻译控制和 mRNA 的稳定。
