Schneider J, Korn-Wendisch F, Kutzner H J
Institut für Mikrobiologie, Technische Hochschule Darmstadt, FRG.
J Gen Microbiol. 1990 Apr;136(4):767-72. doi: 10.1099/00221287-136-4-767.
Three species-specific, temperate actinophages of Streptomyces coelicolor Müller, phi SC623, phi SC347 and phi SC681, were compared with respect to host range, virion structure, antiserum cross-inactivation, DNA-restriction pattern, DNA hybridization, and DNA base composition. The restriction map of phi SC623 (57 kb) was established with eight restriction enzymes; the homologies of this phage with phi SC347 and phi SC681 suggested that it might be a hybrid phage composed of approximately equal parts homologous to one of the other two phages. No homology was detected between phi SC623 and R4, a temperate, wide-host-range phage which can also lysogenize S. coelicolor Müller.
对天蓝色链霉菌Müller的三种种特异性温和肌动噬菌体phi SC623、phi SC347和phi SC681,在宿主范围、病毒粒子结构、抗血清交叉失活、DNA限制图谱、DNA杂交以及DNA碱基组成方面进行了比较。用8种限制酶构建了phi SC623(57 kb)的限制图谱;该噬菌体与phi SC347和phi SC681的同源性表明,它可能是一种杂种噬菌体,由与其他两种噬菌体之一同源的大致相等部分组成。在phi SC623与R4(一种也能使天蓝色链霉菌Müller溶源化的温和、广宿主范围噬菌体)之间未检测到同源性。