Sladkova I A, Chinenova T A, Pel'ts L B, Rebentish B A, Lomovskaia N D
Mol Biol (Mosk). 1981 Sep-Oct;15(5):1051-8.
As shown by genetical and physical methods, the total preparation of phiC43 phage obtained after spontaneous induction of the prophage from S. lividans 803 strain is a heterogenous population. The wild-type phage (phi C43 wt) is only represented in 5--10% of the population. The majority of phage variants are not able to establish the lysogenic state. The structure of DNA molecules of some phages from the total preparations was characterized by electron microscopy of DNA heteroduplexes. Molecules of phiC43 wt DNA appeared to be completely homologous to those of recently studied phiC62 phage, except for two small regions of approximately 0.3 kb in the central part. Phage variants defective in establishment of the lysogenic state were distributed to two groups. One of them consists of deletion variants, the other--deletion/insertion variants. Deletions in DNA molecule of all nonlysogenizing phage overlap. The region of overlapping seems to be responsible for establishment of the lysogenic state. In the same region, deletion of DNA molecules of mutant phiC311 yg2 has been located. Three deletion/insertion variants contain homologous foreign sequences of various length. It is likely that these insertions are fragments of the host chromosomal DNA.
通过遗传学和物理方法表明，从变铅青链霉菌803菌株中前噬菌体自发诱导后获得的phiC43噬菌体总制剂是一个异质群体。野生型噬菌体（phi C43 wt）仅占群体的5%-10%。大多数噬菌体变体无法建立溶原状态。通过DNA异源双链体的电子显微镜对总制剂中一些噬菌体的DNA分子结构进行了表征。phiC43 wt DNA分子似乎与最近研究的phiC62噬菌体的DNA分子完全同源，除了中部大约0.3 kb的两个小区域。在建立溶原状态方面有缺陷的噬菌体变体分为两组。其中一组由缺失变体组成，另一组由缺失/插入变体组成。所有非溶原性噬菌体的DNA分子中的缺失相互重叠。重叠区域似乎负责溶原状态的建立。在同一区域，已定位了突变体phiC311 yg2的DNA分子缺失。三个缺失/插入变体包含不同长度的同源外源序列。这些插入很可能是宿主染色体DNA的片段。
