Miquel Jordi, Vilavella Daniel, Swiderski Zdzisław, Shimalov Vladimir V, Torres Jordi
Laboratori de Parasitologia, Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII, sn, 08028 Barcelona, Spain - Institut de Recerca de la Biodiversitat, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain.
Parasite. 2013;20:28. doi: 10.1051/parasite/2013028. Epub 2013 Aug 29.
The present work constitutes the first ultrastructural analysis of the spermatozoon in the Pleurogenidae, with the study of three species belonging to three of the 16 genera included in this family, namely Pleurogenes claviger, Pleurogenoides medians and Prosotocus confusus. The mature spermatozoa of these pleurogenids present two axonemes of the 9+"1" trepaxonematan pattern, a nucleus, two mitochondria, two bundles of parallel cortical microtubules, external ornamentation, spine-like bodies and granules of glycogen. The organization of these characters in the sperm cell is similar in the three species. Thus, the anterior spermatozoon extremity is filiform and a continuous and submembranous layer of parallel cortical microtubules surrounds the axonemes at their anterior end. The posterior spermatozoon extremity exhibits the second axoneme and corresponds to the Cryptogonimidean type of Quilichini et al. (2010). Slight differences were noted between the spermatozoon of P. confusus and those of the two remaining species in the location of mitochondria.
本研究首次对侧殖科精子进行了超微结构分析，研究了该科16个属中的3个属的3个物种，即棒形侧殖吸虫、中型拟侧殖吸虫和混淆前殖吸虫。这些侧殖科动物的成熟精子具有9 + “1” 型的双鞭毛，一个细胞核、两个线粒体、两束平行的皮质微管、外部纹饰、棘状小体和糖原颗粒。这三个物种精子细胞中这些特征的组织方式相似。因此，精子前端呈丝状，一束连续的、位于膜下的平行皮质微管在轴丝前端环绕着它们。精子后端有第二条轴丝，与Quilichini等人（2010年）描述的隐殖亚目类型相对应。在混淆前殖吸虫的精子与其余两个物种的精子之间，线粒体的位置存在细微差异。
