Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, People's Republic of China; Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China; Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing 210037, People's Republic of China.
Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, People's Republic of China; Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China; Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing 210037, People's Republic of China.
Bioresour Technol. 2019 Aug;285:121322. doi: 10.1016/j.biortech.2019.121322. Epub 2019 Apr 4.
Soluble arabinogalactan (AG) in larch leads to reagent waste during its biorefining using oxidative pretreatment strategies. A two-stage pretreatment of water pre-extraction followed by alkaline hydrogen peroxide (AHP) pretreatment was investigated to more efficiently convert larch cellulose into glucose, while also obtaining a value-added AG product stream. The results showed that water pre-extraction increases the lignin selectivity of both NaOH and HO reagents, translating to improved lignin removal and enzymatic hydrolysis yields. This was found to be related to cellulose accessibility alongside the effective consumption of the reagents. Moreover, the addition of mannanase also significantly enhanced enzymatic digestibility of pretreated solid from 81.0% to 97.7% (4% HO charge and 180 °C) when 40 U/g mannanase was supplemented with 20 FPU/g cellulase. In all, it was demonstrated that coupling mannanase with cellulase could improve larch's enzymatic digestibility and overall viability for biorefining processes.
落叶松中的可溶性阿拉伯半乳聚糖（AG）在使用氧化预处理策略进行生物炼制时会导致试剂浪费。研究了水预提取 followed by 碱性过氧化氢（AHP）预处理的两段预处理方法，以更有效地将落叶松纤维素转化为葡萄糖，同时获得增值的 AG 产物流。结果表明，水预提取增加了 NaOH 和 HO 试剂的木质素选择性，从而提高了木质素去除率和酶水解产率。这被认为与纤维素的可及性以及试剂的有效消耗有关。此外，当添加 40 U/g 甘露聚糖酶和 20 FPU/g 纤维素酶时，添加甘露聚糖酶还显著提高了预处理固体的酶解消化率，从 81.0%提高到 97.7%（4% HO 用量和 180°C）。总之，结果表明，甘露聚糖酶与纤维素酶的结合可以提高落叶松的酶解消化率和生物炼制过程的整体可行性。
