Microbial Resources and Application Laboratory, College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
Carbohydr Polym. 2013 Oct 15;98(1):922-30. doi: 10.1016/j.carbpol.2013.07.014. Epub 2013 Jul 12.
The extracellular polysaccharide (LEP) produced by Lachnum YM281 was obtained from the fermentation broth, and LEP-1b with molecular weight of 4.02×10(4) Da was separated and sequentially purified through DEAE-cellulose 52 column chromatography and Sepharose CL-6B column chromatography. GC-MS, IR and NMR ((1)H, (13)C) spectroscopy analysis indicated that the repeat unit of LEP-1b was: [formula; see text] The effects of LEP-1b on the serum lipids, liver lipids levels and aminotransferase activities of model mice with hyperlipidemic fatty live were studied, and the results showed that LEP-1b had strong lipid lowering and liver protecting effects on mice with hyperlipidemic fatty live.
由 Lachnum YM281 产生的胞外多糖（LEP）从发酵液中获得，分子量为 4.02×10(4) Da 的 LEP-1b 通过 DEAE-纤维素 52 柱层析和 Sepharose CL-6B 柱层析进行分离和顺序纯化。GC-MS、IR 和 NMR ((1)H、(13)C)光谱分析表明，LEP-1b 的重复单元为：[公式；见正文] 研究了 LEP-1b 对高脂脂肪肝模型小鼠血清脂质、肝脂质水平和转氨酶活性的影响，结果表明，LEP-1b 对高脂脂肪肝小鼠具有很强的降血脂和保肝作用。
