从 Lachnum YM281 中提取多糖的纯化、结构、降血脂和保肝作用。
Purification, structure, lipid lowering and liver protecting effects of polysaccharide from Lachnum YM281.
机构信息
Microbial Resources and Application Laboratory, College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
出版信息
Carbohydr Polym. 2013 Oct 15;98(1):922-30. doi: 10.1016/j.carbpol.2013.07.014. Epub 2013 Jul 12.
The extracellular polysaccharide (LEP) produced by Lachnum YM281 was obtained from the fermentation broth, and LEP-1b with molecular weight of 4.02×10(4) Da was separated and sequentially purified through DEAE-cellulose 52 column chromatography and Sepharose CL-6B column chromatography. GC-MS, IR and NMR ((1)H, (13)C) spectroscopy analysis indicated that the repeat unit of LEP-1b was: [formula; see text] The effects of LEP-1b on the serum lipids, liver lipids levels and aminotransferase activities of model mice with hyperlipidemic fatty live were studied, and the results showed that LEP-1b had strong lipid lowering and liver protecting effects on mice with hyperlipidemic fatty live.
由 Lachnum YM281 产生的胞外多糖（LEP）从发酵液中获得，分子量为 4.02×10(4) Da 的 LEP-1b 通过 DEAE-纤维素 52 柱层析和 Sepharose CL-6B 柱层析进行分离和顺序纯化。GC-MS、IR 和 NMR ((1)H、(13)C)光谱分析表明，LEP-1b 的重复单元为：[公式；见正文] 研究了 LEP-1b 对高脂脂肪肝模型小鼠血清脂质、肝脂质水平和转氨酶活性的影响，结果表明，LEP-1b 对高脂脂肪肝小鼠具有很强的降血脂和保肝作用。
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