Surhio Maheen Mahwish, Wang Yufen, Xu Ping, Shah Faisal, Li Jinglei, Ye Ming
Microbial Resources and Application Laboratory, College of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
Int J Biol Macromol. 2017 Jun;99:88-95. doi: 10.1016/j.ijbiomac.2017.01.148. Epub 2017 Feb 16.
Hyperlipidemia is one of the major health problem people suffering throughout the world, which is also a major risk factor for chronic heart disease that can lead to death. The current study was designed to investigate antihyperlipidemic and hepatoprotective effects of selenium modified exopolysaccharide of Lachnum sp. (SeLEP-1b) on high-fat induced mice model. Nitric acid-sodium selenite (HNO-NaSeO) method was used to selenize pure LEP-1b. FT-IR and C NMR results confirmed the modification of LEP-1b into SeLEP-1b. The main structure of SeLEP-1b was similar to the original structure of native LEP-1b and Se(O)OH group was attached to the O-6 position of C-6 in LEP-1b. Oral administration of LEP-1b and SeLEP-1b (200mg/kg) notably reduced the serum and liver lipids, atherogenic index, and enhanced the activities of antioxidant enzymes of hyperlipidemic mice, aswell improved the histopathological status of hepatic tissues. Hence, SeLEP-1b showed better effects than LEP-1b at the same doses. SeLEP-1b demonstrated promising lipid-lowering and liver protecting activities, which may be considered as a novel compound to treat hyperlipidemia and also act as a hepatoprotective agent.
高脂血症是全球人们面临的主要健康问题之一，也是导致死亡的慢性心脏病的主要危险因素。本研究旨在探讨拉氏菌属硒修饰胞外多糖（SeLEP-1b）对高脂诱导小鼠模型的抗高脂血症和肝脏保护作用。采用硝酸-亚硒酸钠（HNO-NaSeO）法对纯LEP-1b进行硒化。傅里叶变换红外光谱（FT-IR）和碳核磁共振（C NMR）结果证实LEP-1b已被修饰为SeLEP-1b。SeLEP-1b的主要结构与天然LEP-1b的原始结构相似，且Se（O）OH基团连接在LEP-1b中C-6的O-6位上。口服LEP-1b和SeLEP-1b（200mg/kg）可显著降低高脂血症小鼠的血清和肝脏脂质、动脉粥样硬化指数，并增强抗氧化酶的活性，同时改善肝组织的组织病理学状态。因此，相同剂量下SeLEP-1b的效果优于LEP-1b。SeLEP-1b具有良好的降脂和肝脏保护活性，可被视为一种治疗高脂血症的新型化合物，同时也可作为一种肝脏保护剂。
