Tomas Bata University in Zlín, Faculty of Technology, Department of Physics and Material Engineering, nám. T.G. Masaryka 275, 760 01 Zlín, Czech Republic.
Carbohydr Polym. 2013 Oct 15;98(1):1039-44. doi: 10.1016/j.carbpol.2013.06.057. Epub 2013 Jul 11.
Interaction of hyaluronan (NaHy) with the quaternary salt, benzalkonium chloride (BAC), was studied. Based on the DLS experiments, viscometry and surface tension measurements executed on hyaluronan samples with two molecular weights of Mw=1.8 MDa and Mw=0.35 MDa, the hypothesis was proposed suggesting that at certain BAC concentrations, hyaluronan can form aggregates, which lead to increase of the polymer coil size measured as z-average diameter. Moreover, it was confirmed that within the whole range of BAC concentrations, repeated variations in size and conformations of polymer coils occur, being connected with the critical micellar concentration of BAC and with hydrophobic interactions of non-polar segments of BAC with hyaluronan hydrophobic domains. Tensiometry, DLS and viscometry data support the assumption that variations of thermodynamical "favourability" of BAC-BAC or NaHy-BAC interactions take place in hyaluronan solutions, based on the BAC concentration.
研究了透明质酸（NaHy）与季铵盐苯扎氯铵（BAC）的相互作用。根据 DLS 实验、两种分子量为 Mw=1.8 MDa 和 Mw=0.35 MDa 的透明质酸样品的粘度和表面张力测量，提出了一个假设，即在一定的 BAC 浓度下，透明质酸可以形成聚集体，导致聚合物线圈尺寸（以 z 均直径测量）增加。此外，还证实了在整个 BAC 浓度范围内，聚合物线圈的尺寸和构象会反复发生变化，这与 BAC 的临界胶束浓度以及 BAC 的非极性部分与透明质酸疏水区之间的疏水相互作用有关。张力计、DLS 和粘度计数据支持了这样的假设，即在基于 BAC 浓度的透明质酸溶液中，BAC-BAC 或 NaHy-BAC 相互作用的热力学“有利性”发生变化。
