Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon 305-764, South Korea.
Carbohydr Polym. 2013 Oct 15;98(1):1179-85. doi: 10.1016/j.carbpol.2013.07.028. Epub 2013 Jul 18.
In this study, methylcellulose (MC) was used to control the gelation time of silk fibroin (SF) aqueous solution. The gelation time was measured using a Vibro Viscometer at 50 °C. Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and a texture meter were used to investigate the effect of MC on the hydrogelation of SF solution. SF/MC hydrogels could be formed by the addition of MC, although their gelation time was increased with MC content. To examine the conformational change of SF/MC hydrogels, time-resolved FT-IR spectra were obtained at constant temperature using a custom-made IR chamber. From FT-IR spectra focused on the amide I peak position, the transition of SF molecules in SF/MC solution from a random coil to a β-sheet structure was inhibited in the presence of MC molecules. In addition, the drug release of SF/MC hydrogels loaded with 5-aminosalicylic acid was studied in 2-dimensional (2-D) and 3-dimensional (3-D) conditions in vitro. The drug release behavior of SF or SF/MC hydrogels was measured using UV-Vis spectroscopy. The release rate of 5-aminosalicylic acid in SF/MC hydrogel was lower than that of SF hydrogel, which may be closely associated with the hydrophilic interaction between MC and 5-aminosalicylic acid. This approach to controlling the sol-gel transition and the drug release of SF hydrogels by the addition of MC will be useful in the design and tailoring of novel materials for biomedical applications.
在这项研究中，甲基纤维素（MC）被用于控制丝素蛋白（SF）水溶液的胶凝时间。在 50°C 下使用 Vibro 黏度计测量胶凝时间。傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）和质构仪用于研究 MC 对 SF 溶液水凝胶化的影响。尽管 MC 含量的增加会延长 SF/MC 水凝胶的胶凝时间，但通过添加 MC 可以形成 SF/MC 水凝胶。为了研究 SF/MC 水凝胶的构象变化，在恒温下使用定制的红外腔获得了时间分辨的 FT-IR 光谱。从聚焦在酰胺 I 峰位置的 FT-IR 光谱可以看出，在 MC 分子存在的情况下，SF 分子在 SF/MC 溶液中的构象从无规线团转变为β-折叠结构的转变受到抑制。此外，研究了载有 5-氨基水杨酸的 SF/MC 水凝胶在二维（2-D）和三维（3-D）条件下的体外药物释放。使用紫外可见光谱法测量 SF 或 SF/MC 水凝胶的药物释放行为。SF/MC 水凝胶中 5-氨基水杨酸的释放速率低于 SF 水凝胶，这可能与 MC 和 5-氨基水杨酸之间的亲水性相互作用密切相关。通过添加 MC 来控制 SF 水凝胶的溶胶-凝胶转变和药物释放的这种方法将有助于设计和定制用于生物医学应用的新型材料。
