Kim Min Hee, Kim Beom Su, Lee Jun, Cho Donghwan, Kwon Oh Hyeong, Park Won Ho
Department of Advanced Organic Materials and Textile Engineering System, College of Engineering, Chungnam National University, Daejeon, 34134 South Korea.
Wonkwang Bone Regeneration Institute, Wonkwang University, Iksan, South Korea.
Biomater Res. 2017 Jun 24;21:12. doi: 10.1186/s40824-017-0098-2. eCollection 2017.
In this study, silk fibroin (SF) composite hydrogels containing hydroxyapatite (HAP) nanoparticles (NPs) for bone tissue engineering were fabricated using gamma-ray (γ-ray) irradiation treatment. During the irradiation, the HAP dispersed SF solution was changed to the chemically crosslinked SF hydrogel.
Distribution of HAP NPs in the SF hydrogel was examined by SEM imagery and energy dispersive X-ray spectrophotometry, and the crystalline structure of SF composite hydrogels was also confirmed by X-ray diffractometry. An optimum preparation condition of the SF/HAP composite hydrogels was determined with various HAP contents. For evaluation of the osteogenic differentiation of human mesenchymal stem cells (hMSCs), alkaline phosphatase activity (ALP), HAP nucleation in SBF and in vitro calcium accumulation were measured.
The results revealed that compared with the pure SF hydrogels, the SF/HAP composite hydrogels improved osteogenic differentiation.
This paper demonstrates the great potential of the SF/HAP composite hydrogels in terms of the production of the bone tissue engineering scaffolds for which osteogenesis is required.
在本研究中，采用伽马射线（γ射线）辐照处理制备了含羟基磷灰石（HAP）纳米颗粒（NPs）的丝素蛋白（SF）复合水凝胶用于骨组织工程。在辐照过程中，HAP分散的SF溶液转变为化学交联的SF水凝胶。
通过扫描电子显微镜图像（SEM）和能量色散X射线光谱法检测HAP NPs在SF水凝胶中的分布，并通过X射线衍射法确认SF复合水凝胶的晶体结构。用不同的HAP含量确定SF/HAP复合水凝胶的最佳制备条件。为评估人间充质干细胞（hMSCs）的成骨分化，测量了碱性磷酸酶活性（ALP）、模拟体液（SBF）中的HAP成核以及体外钙积累情况。
结果表明，与纯SF水凝胶相比，SF/HAP复合水凝胶改善了成骨分化。
本文证明了SF/HAP复合水凝胶在生产需要成骨的骨组织工程支架方面具有巨大潜力。
