Department of Food Science and Technology , Chung-Ang University , Anseong 17546 , Korea.
Korea Food Research Institute , Jeonbuk 55365 , Korea.
J Agric Food Chem. 2018 Aug 1;66(30):8079-8085. doi: 10.1021/acs.jafc.8b02599. Epub 2018 Jul 20.
We enzymatically prepared structured monogalactosydiacylglycerols (MGDGs) enriched in pinolenic acid (PLA). PLA-enriched free fatty acids (FFAs) containing ∼86 mol % PLA were produced from an FFA fraction obtained from pine nut oil (PLA content, ∼13 mol %) by urea crystallization. Commercial MGDGs (5 mg) were acidolyzed with PLA-enriched FFAs using four commercial immobilized lipases as biocatalysts. The reaction was performed in acetone (4 mL) in a stirred-batch reactor. Lipozyme RM IM (immobilized Rhizomucor miehei lipase) was the most effective biocatalyst for the reaction. Structured MGDGs containing 42.1 mol % PLA were obtained under optimal reaction conditions: temperature, 25 °C; substrate molar ratio, 1:30 (MGDGs/PLA-enriched FFAs); enzyme loading, 20 wt % of total substrates; and reaction time, 36 h. The structured MGDGs were separated from the reaction products at a purity of 96.6 wt % using silica column chromatography. The structured MGDGs could be possibly used as emulsifiers with appetite-suppression effects.
我们通过酶法制备富含松烯酸（PLA）的结构单半乳糖二酰基甘油（MGDG）。通过尿素结晶从松仁油（PLA 含量约为 13 mol%）的脂肪酸（FFA）馏分中获得富含 PLA 的游离脂肪酸（FFA），其含有约 86 mol%的 PLA。用 PLA 富集的 FFA 通过酸解 5mg 商业 MGDG，使用四种商业固定化脂肪酶作为生物催化剂。反应在搅拌分批反应器中在丙酮（4 mL）中进行。Lipozyme RM IM（固定化米黑根毛霉脂肪酶）是该反应最有效的生物催化剂。在最佳反应条件下获得了含有 42.1 mol% PLA 的结构 MGDG：温度为 25°C；底物摩尔比为 1:30（MGDG/PLA 富集 FFA）；酶用量为总底物的 20wt%；反应时间为 36 小时。使用硅胶柱色谱法将结构 MGDG 从反应产物中分离出来，纯度为 96.6wt%。结构 MGDG 可能可用作具有抑制食欲作用的乳化剂。
