Laboratorio de Biología Molecular, Instituto Tecnológico de Tlajomulco, Jalisco (ITTJ), km 10 Carretera a San Miguel Cuyutlán, CP 45640 Tlajomulco de Zúñiga, Jalisco, Mexico.
Unidad de Biotecnología e Ingeniería Genética de Plantas, Cinvestav-Unidad Irapuato, km 9.6, Libramiento Norte Carretera Irapuato-León, CP 36821 Irapuato, Guanajuato, Mexico.
J Plant Physiol. 2014 Feb 15;171(3-4):359-72. doi: 10.1016/j.jplph.2013.08.002. Epub 2013 Aug 27.
The expression of genes coding for sucrose:sucrose 1-fructosyltransferase (1-SST; EC 2.4.1.99) and fructan:fructan 1-fructosyltransferase (1-FFT; EC 2.4.1.100), both fructan biosynthesizing enzymes, characterization by TLC and HPAEC-PAD, as well as the quantification of the fructo-oligosaccharides (FOS) accumulating in response to the exogenous application of sucrose, kinetin (cytokinin) or other plant hormones associated with (a)biotic stress responses were determined in two Agave species grown in vitro, domesticated Agave tequilana var. azul and wild A. inaequidens. It was found that elicitors such as salicylic acid (SA), and jasmonic acid methyl ester (MeJA) had the strongest effect on fructo-oligosaccharide (FOS) accumulation. The exogenous application of 1mM SA induced a 36-fold accumulation of FOS of various degrees of polymerization (DP) in stems of A. tequilana. Other treatments, such as 50mM abscisic acid (ABA), 8% Sucrose (Suc), and 1.0 mg L(-1) kinetin (KIN) also led to a significant accumulation of low and high DP FOS in this species. Conversely, treatment with 200 μM MeJA, which was toxic to A. tequilana, induced an 85-fold accumulation of FOS in the stems of A. inaequidens. Significant FOS accumulation in this species also occurred in response to treatments with 1mM SA, 8% Suc, and 10% polyethylene glycol (PEG). Maximum yields of 13.6 and 8.9 mg FOS per g FW were obtained in stems of A. tequilana and A. inaequidens, respectively. FOS accumulation in the above treatments was tightly associated with increased expression levels of either the 1-FFT or the 1-SST gene in tissues of both Agave species.
蔗糖 1-果糖基转移酶（1-SST；EC 2.4.1.99）和果聚糖：果聚糖 1-果糖基转移酶（1-FFT；EC 2.4.1.100）的基因表达，这两种都是果糖基转移酶，通过 TLC 和 HPAEC-PAD 进行表征，以及对外源蔗糖、激动素（细胞分裂素）或其他与（生物）胁迫反应相关的植物激素处理后在体外生长的两种龙舌兰属植物中积累的果寡糖（FOS）的定量，发现在水杨酸（SA）和茉莉酸甲酯（MeJA）等诱导子对果寡糖（FOS）积累的影响最强。1mM SA 的外源应用诱导 A.tequilana 茎中各种聚合度（DP）的 FOS 积累增加 36 倍。其他处理，如 50mM 脱落酸（ABA）、8%蔗糖（Suc）和 1.0mg L(-1)激动素（KIN）也导致该物种中低和高 DP FOS 的显著积累。相反，200μM MeJA 处理对 A.tequilana 有毒，诱导 A.inaequidens 茎中 FOS 积累增加 85 倍。1mM SA、8% Suc 和 10%聚乙二醇（PEG）处理也导致该物种中 FOS 的显著积累。A.tequilana 和 A.inaequidens 茎中分别获得了 13.6 和 8.9mg FOS/g FW 的最大产量。在上述处理中，FOS 的积累与两种龙舌兰属植物组织中 1-FFT 或 1-SST 基因表达水平的增加密切相关。
