Márquez-López Ruth E, Santiago-García Patricia Araceli, López Mercedes G
Instituto Politécnico Nacional, Centro Interdisciplinario de Investigación Para el Desarrollo Integral Regional-Unidad Oaxaca, Oaxaca 71230, Mexico.
Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN-Unidad Irapuato, Guanajuato 36824, Mexico.
Plants (Basel). 2022 Jul 13;11(14):1834. doi: 10.3390/plants11141834.
Despite the recognition of Weber var. Azul as raw material for producing tequila and obtaining prebiotics, there are other highly relevant species in Mexico. Oaxaca contains a startlingly diverse range of species; Haw. and Zucc. are two classic specimens with great commercial potential. In this study, we examined the fructan fluctuation in these two species during their lifetime in the field (from 1 to 6 years old). First, we analyzed their morphological diversity based on vegetative characteristics. Subsequently, fructan extracts were analyzed by TLC, FT-IR, and HPAEC-PAD to identify carbohydrates. Multivariate analyses of the morphological parameters indicated a morphological divergence between the two species. Furthermore, we found that the concentration of simple carbohydrates and fructans, as well as the fructan DP, changed during plant development. Glucose, fructose, and fructooligosaccharides (FOS) were more abundant in , while showed a greater amount of sucrose and fructans with a high DP. Fructan DP heatmaps were constructed using HPAEC-PAD profiles-the heatmaps were very helpful for establishing an easy correlation between age and the carbohydrate types present in the fructan extracts. This study is an important contribution to the agave fructan knowledge of the Mexican agave diversity.
尽管龙舌兰变种阿苏尔被公认为是生产龙舌兰酒和获取益生元的原料，但墨西哥还有其他高度相关的物种。瓦哈卡州拥有种类惊人的多样物种；霍氏龙舌兰和苏氏龙舌兰是两种具有巨大商业潜力的典型标本。在本研究中，我们考察了这两个物种在田间生长周期（1至6岁）内果聚糖的波动情况。首先，我们基于营养特征分析了它们的形态多样性。随后，通过薄层色谱法（TLC）、傅里叶变换红外光谱法（FT-IR）和高效阴离子交换色谱-脉冲安培检测法（HPAEC-PAD）对果聚糖提取物进行分析以鉴定碳水化合物。对形态参数的多变量分析表明这两个物种之间存在形态差异。此外，我们发现单糖和果聚糖的浓度以及果聚糖的聚合度在植物发育过程中发生了变化。葡萄糖、果糖和低聚果糖（FOS）在[此处原文缺失相关物种名称]中含量更高，而[此处原文缺失相关物种名称]则含有更多的蔗糖和高聚合度果聚糖。利用HPAEC-PAD图谱构建了果聚糖聚合度热图——这些热图对于在年龄与果聚糖提取物中存在的碳水化合物类型之间建立简单的关联非常有帮助。这项研究对墨西哥龙舌兰多样性的龙舌兰果聚糖知识做出了重要贡献。
