Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A. C, Av. Normalistas No 800, Col. Colinas de la Normal, 44270 Guadalajara, Jalisco, Mexico.
Food Funct. 2013 Aug;4(8):1237-44. doi: 10.1039/c3fo60083a.
Fructans are dietary fibers with beneficial effects on the gastrointestinal physiology and offer a promising approach for the treatment of some metabolic disorders associated with obesity. In vitro and in vivo studies were developed to test the safety of fructans obtained from Agave tequilana Weber var. azul. Additionally, an in vivo experiment using a diet-induced obesity model was performed to compare the effect of agave fructans with different degree of polymerization (DP) profiles: agave fructans with DP > 10 (LcF), agave FOS with DP < 10 (ScF), and agave fructans with and without demineralization (dTF, TF) versus commercial chicory fructans (OraftiSynergy1™) on the body weight change, fat, total cholesterol, triglycerides and count of fecal Lactobacillus spp. and Bifidobacterium spp. Results showed that A. tequilana fructans were not mutagenic and were safe even at a dose of 5 g per kg b.w. Obese mice that received ScF showed a significant decrease in body weight gain, fat tissue and total cholesterol without increasing the count of fecal Bifidobacteria. Whereas, obese mice that received LcF and TF showed decreased triglycerides and an increased count of fecal Bifidobacteria. Interestingly, although obese mice that received dTF did not show changes in body weight gain, fat tissue, total cholesterol or triglycerides, they showed an increase in the count of Bifidobacteria. These results demonstrate that both the degree of polymerization and the demineralization process can influence the biological activity of agave fructans.
果聚糖是膳食纤维的一种，对胃肠道生理具有有益作用，并为治疗与肥胖相关的某些代谢紊乱提供了一种有前途的方法。开展了体外和体内研究来测试从龙舌兰属韦伯 var. 蓝获得的果聚糖的安全性。此外，还进行了一项使用饮食诱导肥胖模型的体内实验，以比较具有不同聚合度（DP）分布的龙舌兰果聚糖的效果：聚合度> 10（LcF）的龙舌兰果聚糖、聚合度< 10 的龙舌兰低聚果糖（ScF）、以及有和无脱矿化的龙舌兰果聚糖（dTF、TF）与商业菊苣果聚糖（OraftiSynergy1™）对体重变化、脂肪、总胆固醇、甘油三酯和粪便乳酸菌属和双歧杆菌属数量的影响。结果表明，龙舌兰属果聚糖没有致突变性，即使在 5 g/kg bw 的剂量下也是安全的。接受 ScF 的肥胖小鼠体重增加、脂肪组织和总胆固醇显著减少，而粪便双歧杆菌数量没有增加。相比之下，接受 LcF 和 TF 的肥胖小鼠甘油三酯降低，粪便双歧杆菌数量增加。有趣的是，尽管接受 dTF 的肥胖小鼠体重增加、脂肪组织、总胆固醇或甘油三酯没有变化，但它们的双歧杆菌数量增加。这些结果表明，聚合度和脱矿化过程都可以影响龙舌兰果聚糖的生物学活性。
