Adalı Terin
Department of Biomedical Engineering, Faculty of Engineering, Near East University, Lefkosa, Turkey.
Biomed Mater Eng. 2013;23(5):349-59. doi: 10.3233/BME-130759.
Poly(methacrylates), namely 2-hydroxy ethyl methacrylate (HEMA), ethylene glycol dimethacrylate (EGDMA) and triethylene glycol dimethacrylate (TEGDMA) were grafted onto chitosan by using ceric ammonium nitrate as a redox initiator. Semi-IPN gels of chitosan-graft-poly(HEMA)-graft-poly(EGDMA) and chitosan-graft-poly(HEMA)-graft-poly(TEGDMA) were obtained. The grafting conditions were optimized with respect to monomer concentrations. The products were characterized by TGA, FTIR, XRD and SEM techniques. The solubility of the grafted products in aqueous medium decreased with increasing grafting percentage. The insoluble gels exhibited a highly pH sensitive swelling behaviour. TGA thermograms showed that poly(HEMA)/poly(TEGDMA)-grafted product is much more stable than poly(HEMA)/poly(EGDMA)-grafted product showing that TEGDMA is a more effective crosslinker than EGDMA. According to XRD analysis TEGDMA has a higher tendency to form ordered structures than EGDMA as it is capable of chain folding. The results of cytotoxicity studies revealed that the methacrylate-grafted chitosans were noncytotoxic and good candidates for biomedical applications.
以硝酸铈铵作为氧化还原引发剂，将聚甲基丙烯酸酯，即甲基丙烯酸 2-羟乙酯（HEMA）、乙二醇二甲基丙烯酸酯（EGDMA）和三乙二醇二甲基丙烯酸酯（TEGDMA）接枝到壳聚糖上。制备了壳聚糖-接枝-聚（HEMA）-接枝-聚（EGDMA）和壳聚糖-接枝-聚（HEMA）-接枝-聚（TEGDMA）的半互穿网络凝胶。针对单体浓度对接枝条件进行了优化。通过热重分析（TGA）、傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）和扫描电子显微镜（SEM）技术对产物进行了表征。接枝产物在水性介质中的溶解度随接枝率的增加而降低。不溶性凝胶表现出高度的 pH 敏感溶胀行为。TGA 热重曲线表明，聚（HEMA）/聚（TEGDMA）接枝产物比聚（HEMA）/聚（EGDMA）接枝产物更稳定，这表明 TEGDMA 是比 EGDMA 更有效的交联剂。根据 XRD 分析，由于 TEGDMA 能够链折叠，其形成有序结构的倾向比 EGDMA 更高。细胞毒性研究结果表明，甲基丙烯酸接枝的壳聚糖无细胞毒性，是生物医学应用的良好候选材料。
