Zhang Shailin, Sun Junying, Xu Ying, Qian Shi, Wang Bing, Liu Fei, Liu Xuanyong
Department of Orthopaedics, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Biomed Mater Eng. 2013;23(5):373-85. doi: 10.3233/BME-130761.
Zirconia films were prepared on titanium by cathodic arc deposition technique. The surface topography and element composition of the films were characterized by scanning electron microscopy and X-ray photoelectron spectroscopy, respectively. Osteoblast-like MG63 cells were cultured on the surface of the zirconia films in vitro, and cell behaviour was investigated, with titanium as control. The results obtained from scanning electron microscopy and immunofluorescence studies showed that the MG63 cells on ZrO2 films spread better than those on Ti. The CCK8 assay indicated that the zirconia films promoted the proliferation of MG63 cells. The results of alkaline phosphatase (ALP) activity test and the expression of osteogenic marker genes, such as ALP, collagen I and osteocalcin, demonstrated that the differentiation of MG63 cells might be enhanced by zirconia films. In addition, the zirconia films possibly regulated osteoclastogenic gene expression by stimulating the expression of osteoprotegerin and reducing the expression of receptor activator of nuclear factor-kappaB ligand. The present work suggests that the ZrO2 film is worth further consideration for orthopedic implant applications.
采用阴极电弧沉积技术在钛表面制备了氧化锆薄膜。分别通过扫描电子显微镜和X射线光电子能谱对薄膜的表面形貌和元素组成进行了表征。在体外将成骨样MG63细胞培养在氧化锆薄膜表面，并以钛作为对照研究细胞行为。扫描电子显微镜和免疫荧光研究结果表明，ZrO2薄膜上的MG63细胞比钛上的细胞铺展得更好。CCK8检测表明氧化锆薄膜促进了MG63细胞的增殖。碱性磷酸酶（ALP）活性测试以及成骨标志物基因如ALP、I型胶原和骨钙素表达的结果表明，氧化锆薄膜可能增强了MG63细胞的分化。此外，氧化锆薄膜可能通过刺激骨保护素的表达和降低核因子-κB受体激活剂配体的表达来调节破骨细胞生成基因的表达。目前的工作表明，ZrO2薄膜在骨科植入物应用方面值得进一步考虑。
