Ozeki K, Goto T, Aoki H, Masuzawa T
Department of Mechanical Engineering, Ibaraki University, Ibaraki, Japan.
Department of Biosciences, Division of Anatomy, Kyushu Dental College, Kitakyushu, Japan.
Biomed Mater Eng. 2014;24(5):1793-802. doi: 10.3233/BME-140990.
Hydroxyapatite (HA) thin films were prepared on a zirconia (ZrO2) substrate using a sputtering technique, and the film was also coated on a titanium (Ti) substrate for comparison. The coated films were recrystallised using a hydrothermal treatment to reduce film dissolution. The films were then characterised by X-ray diffractometry (XRD) and scanning electron microscopy (SEM). The osteocompatiblity of the films was evaluated by investigating the alkaline phosphatase (ALP) activity and the size of the bone formation area of osteoblast cells. In the XRD patterns of the as-sputtered films on the ZrO2 substrate, there are no peaks except for those from the ZrO2 substrate. After the hydrothermal treatment, HA peaks appeared in the patterns. Nanoparticles (less than 20 nm) were observed on the ZrO2 substrates in the SEM images of the as-sputtered films. After the hydrothermal treatment, particles of 20-40 nm were observed on the film, whereas the HA film on the Ti substrate was covered by a larger number of globular particles (20-60 nm). In the osteoblast cell cultures, the ALP activity and bone formation area on the HA films on both the ZrO2 and Ti substrates increased after the hydrothermal treatment of the films, and the values for the ZrO2 substrate were higher than those for the Ti substrate.
采用溅射技术在氧化锆（ZrO₂）基底上制备了羟基磷灰石（HA）薄膜，并将该薄膜也涂覆在钛（Ti）基底上用于比较。对涂覆的薄膜进行水热处理使其再结晶，以减少薄膜溶解。然后通过X射线衍射仪（XRD）和扫描电子显微镜（SEM）对薄膜进行表征。通过研究成骨细胞的碱性磷酸酶（ALP）活性和骨形成面积来评估薄膜的骨相容性。在ZrO₂基底上溅射态薄膜的XRD图谱中，除了来自ZrO₂基底的峰外没有其他峰。水热处理后，图谱中出现了HA峰。在溅射态薄膜的SEM图像中，在ZrO₂基底上观察到了纳米颗粒（小于20nm）。水热处理后，在薄膜上观察到20 - 40nm的颗粒，而Ti基底上的HA薄膜被大量球形颗粒（20 - 60nm）覆盖。在成骨细胞培养中，对薄膜进行水热处理后，ZrO₂和Ti基底上HA薄膜的ALP活性和骨形成面积均增加，且ZrO₂基底的值高于Ti基底。
