Viral Zoonoses, Emerging and Vector-Borne Infections Group, Institute of Virology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria.
Infect Genet Evol. 2013 Dec;20:124-30. doi: 10.1016/j.meegid.2013.08.015. Epub 2013 Aug 26.
Ilesha virus is an arthropod-borne virus belonging to the genus Orthobunyavirus of the Bunyaviridae family. Ilesha virus has been isolated from humans in several African countries, mostly in relation with febrile illness and erythema, though there are reported cases of fatal meningoencephalitis and hemorrhagic fever. In the present study, we report the complete genomic sequence of all three Ilesha virus segments (S, M, L) and characterize the open reading frames. The nucleoprotein encoded by segment S contains 59 conserved orthobunyavirus amino acids putatively critical for protein function. For the polyprotein encoded by segment M, potential proteolytic cleavage sites and N-glycosylation sites as well as conserved cysteines are described in reference to other orthobunyaviruses. Within the C terminal glycoprotein Gc a putative fusion peptide could be localized. In the RNA-dependent RNA polymerase encoded by segment L, all strictly conserved amino acids within the four conserved regions known to be catalytically active are present. Phylogenetic analyses conducted for each Ilesha virus genomic segment confirm the classification of Ilesha virus within the Bunyamwera serogroup of orthobunyaviruses. Ilesha virus segments S and L exhibit highest genetic conservation with Bunyamwera virus and Ngari virus, with maximum sequence identities of 88% for segment S and 82% for segment L. However, the M segment was found to be more diverse with a maximum nucleotide identity of 72% to Bunyamwera serogroup viruses.
伊莱沙病毒是一种虫媒病毒，属于布尼亚病毒科正布尼亚病毒属。伊莱沙病毒已从多个非洲国家的人类中分离出来，主要与发热和红斑有关，但也有报道称其导致了致命的脑膜脑炎和出血热。在本研究中，我们报告了所有三个伊莱沙病毒节段（S、M、L）的完整基因组序列，并对开放阅读框进行了特征描述。节段 S 编码的核蛋白包含 59 个保守的正布尼亚病毒氨基酸，这些氨基酸可能对蛋白质功能至关重要。对于节段 M 编码的多蛋白，描述了潜在的蛋白水解切割位点和 N-糖基化位点以及保守的半胱氨酸，这与其他正布尼亚病毒相对应。在 C 末端糖蛋白 Gc 中可以定位到一个推定的融合肽。在节段 L 编码的 RNA 依赖性 RNA 聚合酶中，所有四个已知具有催化活性的保守区域内的严格保守氨基酸都存在。对每个伊莱沙病毒基因组节段进行的系统发育分析证实了伊莱沙病毒在正布尼亚病毒属的布尼亚韦拉血清群中的分类。伊莱沙病毒的节段 S 和 L 与布尼亚韦拉病毒和尼加拉病毒表现出最高的遗传保守性，节段 S 的最大序列同一性为 88%，节段 L 的最大序列同一性为 82%。然而，M 节段显示出更高的多样性，与布尼亚韦拉血清群病毒的最大核苷酸同一性为 72%。
