Aquino V H, Moreli M L, Moraes Figueiredo L T
Unidade Multidepartamental de Pesquisa em Virologia, Faculdade de Medicina de Ribeirão Preto, USP, Ribeirão Preto, Brasil.
Arch Virol. 2003 Jan;148(1):19-28. doi: 10.1007/s00705-002-0913-4.
We described here the complete nucleotide sequence of the L RNA segment of Oropouche virus (genus Orthobunyavirus, family Bunyaviridae). We found the L RNA segment is 6846 nucleotides long and encodes a putative RNA polymerase of 2250 amino acids. Phylogenetic analysis showed that ORO virus cluster to the Orthobunyavirus genus confirming the serological classification. It also showed that Bunyamwera and California viruses, from the Orthobunyavirus genus, are more closely related to each other than to ORO virus. Sequence comparisons performed between the L proteins of 15 bunyaviruses and the PB1 proteins of 3 influenza viruses revealed that ORO L protein contains the 3 regions characteristic of arenaviruses and bunyaviruses. These comparisons also showed the existence of an additional fourth conserved region in the L protein of bunyaviruses that contains at least two active sites.
我们在此描述了奥罗普切病毒（正布尼亚病毒属，布尼亚病毒科）L RNA片段的完整核苷酸序列。我们发现L RNA片段长6846个核苷酸，编码一个推定的含2250个氨基酸的RNA聚合酶。系统发育分析表明，奥罗病毒聚集在正布尼亚病毒属中，证实了血清学分类。分析还表明，正布尼亚病毒属中的布尼亚姆韦拉病毒和加利福尼亚病毒彼此之间的亲缘关系比它们与奥罗病毒的关系更为密切。对15种布尼亚病毒的L蛋白和3种流感病毒的PB1蛋白进行的序列比较显示，奥罗病毒L蛋白含有沙粒病毒和布尼亚病毒特有的3个区域。这些比较还表明，布尼亚病毒L蛋白中存在一个额外的第四保守区域，该区域至少包含两个活性位点。
