Acajjaoui Samira, Zubieta Chloe
Structural Biology, European Synchrotron Radiation Facility, 6 Rue Jules Horowitz, 38000 Grenoble, France.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Sep;69(Pt 9):997-1000. doi: 10.1107/S174430911302006X. Epub 2013 Aug 19.
In higher plants, the MADS-box genes encode a large family of transcription factors (TFs) involved in key developmental processes, most notably plant reproduction, flowering and floral organ development. SEPALLATA 3 (SEP3) is a member of the MADS TF family and plays a role in the development of the floral organs through the formation of multiprotein complexes with other MADS-family TFs. SEP3 is divided into four domains: the M (MADS) domain, involved in DNA binding and dimerization, the I (intervening) domain, a short domain involved in dimerization, the K (keratin-like) domain important for multimeric MADS complex formation and the C (C-terminal) domain, a largely unstructured region putatively important for higher-order complex formation. The entire K domain along with a portion of the I and C domains of SEP3 was crystallized using high-throughput robotic screening followed by optimization. The crystals belonged to space group P2(1)2(1)2, with unit-cell parameters a = 123.44, b = 143.07, c = 49.83 Å, and a complete data set was collected to 2.53 Å resolution.
在高等植物中，MADS盒基因编码一大类参与关键发育过程的转录因子，其中最显著的是植物繁殖、开花和花器官发育。SEPALLATA 3（SEP3）是MADS转录因子家族的成员，通过与其他MADS家族转录因子形成多蛋白复合物，在花器官发育中发挥作用。SEP3分为四个结构域：参与DNA结合和二聚化的M（MADS）结构域、参与二聚化的短I（间隔）结构域、对多聚体MADS复合物形成很重要的K（角蛋白样）结构域以及在很大程度上无结构的C（C末端）结构域，该结构域推测对高阶复合物形成很重要。使用高通量机器人筛选并优化后，SEP3的整个K结构域以及部分I和C结构域得以结晶。这些晶体属于空间群P2(1)2(1)2，晶胞参数为a = 123.44、b = 143.07、c = 49.83 Å，并收集了完整数据集至2.53 Å分辨率。
