Ekizer Abdullah, Uysal Tancan, Güray Enis, Akkuş Derya
Department of Orthodontics, Faculty of Dentistry, Erciyes University, Kayseri, Turkey,
Lasers Med Sci. 2015 Feb;30(2):779-85. doi: 10.1007/s10103-013-1405-3. Epub 2013 Aug 29.
The aim of this experimental study was to evaluate the effects of light-emitting diode-mediated-photobiomodulation therapy (LPT), on the rate of orthodontic tooth movement (TM) and orthodontically induced root resorption, in rats. Twenty male 12-week-old Wistar rats were separated into two groups (control and LPT) and 50 cN of force was applied between maxillary left molar and incisor with a coil spring. In the treatment group, LPT was applied with an energy density of 20 mW/cm(2) over a period of 10 consecutive days directly over the movement of the first molar teeth area. The distance between the teeth was measured with a digital caliper on days 0 (T0), 10 (T1), and 21 (T2) on dental cast models. The surface area of root resorption lacunae was measured histomorphometrically using digital photomicrographs. Mann-Whitney U and Wilcoxon tests were used for statistical evaluation at p < 0.05 level. TM during two different time intervals (T1-T0 and T2-T1) were compared for both groups and a statistically significant difference was found in the LPT group (p = 0.016). The TM amount at the first time period (1.31 ± 0.36 mm) was significantly higher than the second time period (0.24 ± 0.23 mm) in the LPT group. Statistical analysis showed significant differences between two groups after treatment/observation period (p = 0.017). The magnitude of movement in the treatment group was higher (1.55 ± 0.33 mm) compared to the control group (1.06 ± 0.35 mm). Histomorphometric analysis of root resorption, expressed as a percentage, showed that the average relative root resorption affecting the maxillary molars on the TM side was 0.098 ± 0.066 in the LPT group and 0.494 ± 0.224 in the control group. Statistically significant inhibition of root resorption with LPT was determined (p < 0.001) on the TM side. The LPT method has the potential of accelerating orthodontic tooth movement and inhibitory effects on orthodontically induced resorptive activity.
本实验研究的目的是评估发光二极管介导的光生物调节疗法（LPT）对大鼠正畸牙齿移动（TM）速率和正畸诱导牙根吸收的影响。将20只12周龄的雄性Wistar大鼠分为两组（对照组和LPT组），用螺旋弹簧在上颌左侧磨牙和切牙之间施加50 cN的力。在治疗组中，以20 mW/cm²的能量密度连续10天直接照射第一磨牙移动区域。在第0天（T0）、第10天（T1）和第21天（T2），使用数字卡尺在牙模上测量牙齿之间的距离。使用数字显微照片通过组织形态计量学测量牙根吸收腔的表面积。采用Mann-Whitney U检验和Wilcoxon检验进行统计学评估，显著性水平为p < 0.05。比较了两组在两个不同时间间隔（T1 - T0和T2 - T1）的TM情况，发现LPT组有统计学显著差异（p = 0.016）。LPT组第一个时间段的TM量（1.31±0.36 mm）显著高于第二个时间段（0.24±0.23 mm）。统计分析显示，治疗/观察期后两组之间存在显著差异（p = 0.017）。治疗组的移动幅度（1.55±0.33 mm）高于对照组（1.06±0.35 mm）。以百分比表示的牙根吸收组织形态计量分析表明，LPT组TM侧上颌磨牙的平均相对牙根吸收为0.098±0.066，对照组为0.494±0.224。在TM侧，确定LPT对牙根吸收有统计学显著的抑制作用（p < 0.001）。LPT方法具有加速正畸牙齿移动的潜力，并对正畸诱导的吸收活性有抑制作用。
