Mansouri Vahid, Rezaei-Tavirani Mostafa, Zadeh-Esmaeel Mohammad-Mehdi, Rezaei-Tavirani Sina, Razzaghi Mohammadreza, Okhovatian Farshad, Rostami-Nejad Mohammad, Ahmadzade Alireza
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2019 Fall;10(Suppl 1):S1-S6. doi: 10.15171/jlms.2019.S1. Epub 2019 Dec 1.
The Mechanism of laser therapy and also its safety are 2 important features of the application of different types of lasers in medicine. This study aims to investigate the critically affected genes after the treatment of squamous cell carcinoma patients. The gene expression profiles of 4 squamous cell carcinoma patients that were treated via chemoradiotherapy (CRT) plus the laser and 3 similar patients without laser exposure from Gene Expression Omnibus (GEO) were downloaded and were screened to find critical genes via network analysis. The STRING database, Cytoscape software, and the Clue GO plug-in of Cytoscape software were used. The genes HSX70 and NCC27 were determined as neighbors and HSPA1B, CLIC1, RAB13, PPIF, and LCE3D as hub genes. The over-expression of LCE3D was interpreted as the side effect of laser therapy. Apoptosis and the cell cycle were the dominant biological processes regulated by the HSP molecules in the laser-treated patients. The laser affected the main biological processes and simultaneously issued side effects.
激光治疗的机制及其安全性是不同类型激光在医学应用中的两个重要特征。本研究旨在调查鳞状细胞癌患者接受治疗后受到严重影响的基因。从基因表达综合数据库（GEO）下载了4例接受放化疗（CRT）加激光治疗的鳞状细胞癌患者以及3例未接受激光照射的类似患者的基因表达谱，并通过网络分析筛选以找出关键基因。使用了STRING数据库、Cytoscape软件以及Cytoscape软件的Clue GO插件。确定基因HSX70和NCC27为邻接基因，而HSPA1B、CLIC1、RAB13、PPIF和LCE3D为枢纽基因。LCE3D的过表达被解释为激光治疗的副作用。凋亡和细胞周期是激光治疗患者中由热休克蛋白（HSP）分子调节的主要生物学过程。激光影响主要生物学过程并同时产生副作用。
