Altan A Burcu, Bicakci A Altug, Mutaf H Ilhan, Ozkut Mahmut, Inan V Sevinc
Department of Orthodontics, Faculty of Dentistry, Kocaeli University, Kocaeli, Turkey.
Department of Orthodontics, Faculty of Dentistry, Gaziosmanpaşa University, Tokat, Turkey.
Lasers Med Sci. 2015 Nov;30(8):2067-76. doi: 10.1007/s10103-015-1717-6. Epub 2015 Jan 30.
The aim of this study was to evaluate the preventive and/or reparative effects of low-level laser therapy (LLLT) on orthodontically induced inflammatory root resorption (OIIRR) in rats. Thirty rats were divided into four groups (short-term control (SC), short-term laser (SL), long-term control (LC), long-term laser (LL)). In all groups, the left first molar was moved mesially for 11 days. At the end of this period, the rats in groups SC and SL were killed in order to observe the resorption lacunas and to evaluate whether LLLT had any positive effect on root resorption. The groups LC and LL were remained for a healing period of 14 days in order to observe spontaneous repair of the resorption areas and investigate whether LLLT had reparative effects on root resorption. A Ga-Al-As diode laser (Doris, CTL-1106MX, Warsaw, Poland) with a wavelength of 820 nm was used. In SL group, the first molars were irradiated with the dose of 4.8 J/cm2 (50 mW, 12 s, 0.6 J) on every other day during force application. In LL group, the irradiation period was started on the day of appliance removal and the first molars were irradiated with the dose of 4.8 J/cm2 on every other day for the next 14 days. LLLT significantly increased the number of osteoblasts and fibroblasts, and inflammatory response in SL group in comparison with SC group (P = .001). The amount of resorption did not represent any difference between the two groups (P = .16). In LL group, LLLT significantly increased the number of fibroblasts and decreased the amount of resorption in comparison with LC group (P = .001; P = .02). Both parameters indicating the reparative and the resorptive processes were found to be increased by LLLT applied during orthodontic force load. LLLT applied after termination of the orthodontic force significantly alleyed resorption and enhanced/accelerated the healing of OIIRR. LLLT has significant reparative effects on OIIRR while it is not possible to say that it definitely has a preventive effect.
本研究的目的是评估低强度激光疗法（LLLT）对大鼠正畸诱导性炎性牙根吸收（OIIRR）的预防和/或修复作用。30只大鼠被分为四组（短期对照组（SC）、短期激光组（SL）、长期对照组（LC）、长期激光组（LL））。所有组中，左侧第一磨牙均向近中移动11天。在此期间结束时，处死SC组和SL组的大鼠，以观察吸收陷窝，并评估LLLT对牙根吸收是否有任何积极作用。LC组和LL组保留14天的愈合期，以观察吸收区域的自发修复情况，并研究LLLT对牙根吸收是否有修复作用。使用波长为820nm的Ga-Al-As二极管激光器（Doris，CTL-1106MX，波兰华沙）。在SL组中，在施加力的过程中每隔一天以4.8J/cm²的剂量（50mW，12s，0.6J）照射第一磨牙。在LL组中，照射期从拆除矫治器当天开始，在接下来的14天里每隔一天以4.8J/cm²的剂量照射第一磨牙。与SC组相比，LLLT显著增加了SL组中成骨细胞和成纤维细胞的数量以及炎症反应（P = 0.001）。两组之间的吸收量没有差异（P = 0.16）。与LC组相比，LLLT显著增加了LL组中成纤维细胞的数量并减少了吸收量（P = 0.001；P = 0.02）。发现正畸力加载期间应用的LLLT增加了指示修复和吸收过程的两个参数。正畸力终止后应用的LLLT显著减轻了吸收并增强/加速了OIIRR的愈合。LLLT对OIIRR具有显著的修复作用，虽然不能肯定地说它具有预防作用。
