Turčeková Ľudmila, Antolová Daniela, Reiterová Katarína, Spišák František
Institute of Parasitology SAS, Hlinkova 3, 040 01 Košice, Slovak Republic.
Acta Parasitol. 2013 Sep;58(3):361-6. doi: 10.2478/s11686-013-0154-6. Epub 2013 Aug 29.
The protozoan Toxoplasma gondii is an obligate intracellular parasite that infects a wide range of warm-blooded vertebrates. The data about the occurrence of toxoplasmosis in slaughter pigs in the Slovak Republic are still missing. The aim of our study was to estimate the prevalence of toxoplasmosis in pigs from Slovakia during the period of 2006-2010 by ELISA and PCR methods. In sera of 970 slaughter pigs, 2.16% seropositivity to T. gondii was detected. In tissue samples of seropositive pigs the presence of T. gondii DNA was confirmed. In six monitored Slovak regions the seropositivity varied between 1.11 and 3.48%. The statistically significant differences were recorded between the Košice and Prešov region. The seroprevalence of toxoplasmosis in sows (4.26%) was two times higher than that in slaughter pigs (2.06%) (OR = 2.12; 95% CI = 0.48-9.36). Presence of Toxoplasma gondii in tissues of seropositive pig isolates was confirmed by TGR1E and B1 genes and analysis of DNA polymorphism at SAG2 and ROP1 genes revealed the presence of virulent strain of genotype I in 85.7% of infected pigs and an avirulent strain (genotype II) in 14.3% of pigs.
原生动物刚地弓形虫是一种专性细胞内寄生虫，可感染多种温血脊椎动物。斯洛伐克共和国关于屠宰猪弓形虫病发生情况的数据仍然缺失。我们研究的目的是通过酶联免疫吸附测定（ELISA）和聚合酶链反应（PCR）方法估计2006 - 2010年期间斯洛伐克猪群中弓形虫病的流行率。在970头屠宰猪的血清中，检测到2.16%的猪对刚地弓形虫呈血清阳性。在血清阳性猪的组织样本中证实了刚地弓形虫DNA的存在。在斯洛伐克六个监测地区，血清阳性率在1.11%至3.48%之间变化。在科希策和普雷绍夫地区之间记录到了统计学上的显著差异。母猪弓形虫病的血清流行率（4.26%）是屠宰猪（2.06%）的两倍（比值比=2.12；95%置信区间=0.48 - 9.36）。通过TGR1E和B1基因证实了血清阳性猪分离株组织中存在刚地弓形虫，并且对SAG2和ROP1基因的DNA多态性分析显示，85.7%的感染猪中存在I型毒力株，14.3%的猪中存在无毒力株（II型）。
