Environmental Biotechnology Research Centre, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):3293-3296. doi: 10.1099/ijs.0.064907-0. Epub 2014 Jul 3.
A Gram-stain-positive, aerobic, non-motile, non-spore-forming, cocci-shaped actinobacterium, designated strain EBR4-1-2(T), was isolated from a biofilm reactor in Korea. Comparative 16S rRNA gene sequence studies showed the isolate was clearly affiliated with the class Actinobacteria, and was related most closely to Flaviflexus huanghaiensis H5(T), showing 98.9 % similarity. Cells of strain EBR4-1-2(T) formed yellow colonies on R2A agar, contained MK-9(H4) as the predominant menaquinone, and included C18 : 1ω9c, C16 : 0, C16 : 1ω9c and C14 : 0 as the major fatty acids. The cell-wall peptidoglycan type was A5α (l-Lys-l-Ala-l-Lys-d-Glu). The G+C content of the genomic DNA of strain EBR4-1-2(T) was 65.6 mol%. Thus, the combined genotypic and phenotypic data supported the conclusion that strain EBR4-1-2(T) represents a novel species of the genus Flaviflexus, for which the name Flaviflexus salsibiostraticola sp. nov. is proposed. The type strain is EBR4-1-2(T) ( = KCTC 33148(T) = JCM 19016(T)).
一株革兰氏阳性、需氧、不运动、不产芽孢的球形放线菌，被命名为 EBR4-1-2(T) 菌株，从韩国的生物膜反应器中分离得到。比较 16S rRNA 基因序列的研究表明，该分离株与放线菌纲密切相关，与 Flaviflexus huanghaiensis H5(T) 的相似度最高，为 98.9%。EBR4-1-2(T) 菌株的细胞在 R2A 琼脂上形成黄色菌落，含有 MK-9(H4) 作为主要的类异戊二烯醌，包含 C18 : 1ω9c、C16 : 0、C16 : 1ω9c 和 C14 : 0 作为主要脂肪酸。细胞壁肽聚糖类型为 A5α (l-Lys-l-Ala-l-Lys-d-Glu)。EBR4-1-2(T) 菌株的基因组 DNA G+C 含量为 65.6 mol%。因此，综合基因型和表型数据表明，EBR4-1-2(T) 菌株代表了 Flaviflexus 属的一个新种，建议将其命名为 Flaviflexus salsibiostraticola sp. nov.。该模式菌株为 EBR4-1-2(T)（ = KCTC 33148(T) = JCM 19016(T)）。
