University of El-Manar II, Faculty of Sciences, Unity of Research of Vegetal Ecology, Campus Academia, 2092 Tunis, Tunisia.
Phytochemistry. 2013 Nov;95:197-206. doi: 10.1016/j.phytochem.2013.08.001. Epub 2013 Aug 27.
The medicinal plant Ammi visnaga is a valuable source of furanochromones and pyranocoumarins used as vasodilator agents. Its ability to germinate under unfavourable growth conditions, such as saline soil and hypoxia characterizing clay soils and marshes ecosystems, prompted us to qualitatively characterize secondary metabolites in umbels of A. visnaga plants grown under different conditions (in field, hydroponically controlled, and contrasted by salinity and/or hypoxia) by HPLC-ESI/IT/MS(n) analysis. Subsequently, the quantitative analysis of the bioactive compounds, above all furanochromones and pyranocoumarins, was carried out by HPLC-ESI/QqQ/MS/MS. The results show the influence of growing conditions on the quali-quantitative profile of A. visnaga secondary metabolites and evidence that hydroponic culture leads to increased level of A. visnaga active principles. Furthermore, two furanochromones never reported before were identified and characterized by 1D- and 2D-NMR analysis.
药用植物莳萝茴香是呋喃色酮和吡喃香豆素的重要来源，可用作血管扩张剂。它能够在不利的生长条件下发芽，例如盐渍土和缺氧，这些条件是粘土土壤和沼泽生态系统的特征。这促使我们通过 HPLC-ESI/IT/MS(n)分析定性表征在不同条件（田间、水培控制以及通过盐度和/或缺氧对比）下生长的莳萝茴香植物伞形花序中的次生代谢物。随后，通过 HPLC-ESI/QqQ/MS/MS 进行生物活性化合物（尤其是呋喃色酮和吡喃香豆素）的定量分析。结果表明，生长条件会影响莳萝茴香次生代谢物的质量和数量分布，并证明水培会导致莳萝茴香活性成分水平升高。此外，通过 1D-和 2D-NMR 分析鉴定并表征了两种以前从未报道过的呋喃色酮。
